Detailed information of TA system TA032486

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1584257..1584914
• Replicon: chromosome
• Accession: NZ_AP019007
• Organism: Enterobacter oligotrophicus strain CCA6

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: EoCCA6_RS07470
• Protein ID: WP_152082144.1
• Coordinates: 1584257..1584667 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: V3PWU7
• Locus tag: EoCCA6_RS07475
• Protein ID: WP_010435322.1
• Coordinates: 1584648..1584914 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EoCCA6_RS07450	1580249..1581982	-	1734	WP_152082140.1	single-stranded-DNA-specific exonuclease RecJ	-
EoCCA6_RS07455	1581988..1582701	-	714	WP_152082141.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
EoCCA6_RS07460	1582730..1583626	-	897	WP_152082142.1	site-specific tyrosine recombinase XerD	-
EoCCA6_RS07465	1583728..1584249	+	522	WP_152082143.1	flavodoxin FldB	-
EoCCA6_RS07470	1584257..1584667	-	411	WP_152082144.1	protein YgfX	Toxin
EoCCA6_RS07475	1584648..1584914	-	267	WP_010435322.1	FAD assembly factor SdhE	Antitoxin
EoCCA6_RS07480	1585208..1586188	+	981	WP_152082145.1	tRNA-modifying protein YgfZ	-
EoCCA6_RS07485	1586299..1586958	-	660	WP_152082146.1	hemolysin III family protein	-
EoCCA6_RS07490	1587224..1587955	+	732	WP_152084415.1	MurR/RpiR family transcriptional regulator	-
EoCCA6_RS07495	1588071..1589504	+	1434	WP_152082147.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16157.04 Da
• Isoelectric Point: 11.5020

>T32486 WP_152082144.1 NZ_AP019007:c1584667-1584257 [Enterobacter oligotrophicus]
VVLWQSDLRVSWRSQWMSLLLHGLVAAFVLLMPWPLSYTPLWLLLLSFVVFDSVRSQRRINARQGEIKLLMDSRLRWQGK
EWDIIGTPWMLSVGMMLRLRQVDGGRRQHLWLAADSMDAAEWRDLRRMILQQPTQE

Nucleotide

• Length: 411 bp

>T32486 NZ_AP019007:c1584667-1584257 [Enterobacter oligotrophicus]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTCGCGGC
ATTTGTTTTACTGATGCCGTGGCCGCTTAGCTATACGCCTTTGTGGTTATTGCTGTTGTCATTTGTGGTTTTTGACAGCG
TGCGCAGTCAGCGCCGGATCAATGCCCGTCAGGGCGAAATCAAGCTGCTGATGGACTCTCGCCTGCGCTGGCAGGGGAAA
GAGTGGGATATTATTGGCACGCCGTGGATGCTTAGTGTCGGCATGATGCTTCGGCTACGTCAGGTTGACGGGGGGCGACG
CCAGCATTTGTGGCTGGCGGCAGACAGTATGGATGCTGCAGAGTGGCGAGATTTGCGCCGGATGATACTGCAACAACCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10555.02 Da
• Isoelectric Point: 5.1340

>AT32486 WP_010435322.1 NZ_AP019007:c1584914-1584648 [Enterobacter oligotrophicus]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDSLSDDDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT32486 NZ_AP019007:c1584914-1584648 [Enterobacter oligotrophicus]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCATGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCGTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGACGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: V3PWU7