Detailed information of TA system TA032473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1146124..1146622
• Replicon: chromosome
• Accession: NZ_AP018937
• Organism: Streptococcus pneumoniae strain HU-OH

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EL271_RS06140
• Protein ID: WP_001812387.1
• Coordinates: 1146124..1146378 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B1IC20
• Locus tag: EL271_RS06145
• Protein ID: WP_000208080.1
• Coordinates: 1146380..1146622 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL271_RS06125	1141385..1143853	-	2469	WP_001152997.1	DNA gyrase subunit A	-
EL271_RS06130	1144047..1145033	+	987	WP_000204733.1	L-lactate dehydrogenase	-
EL271_RS06135	1145382..1146077	-	696	WP_000731127.1	potassium channel family protein	-
EL271_RS06140	1146124..1146378	-	255	WP_001812387.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EL271_RS06145	1146380..1146622	-	243	WP_000208080.1	hypothetical protein	Antitoxin
EL271_RS06150	1146713..1147522	-	810	WP_001289493.1	MBL fold metallo-hydrolase	-
EL271_RS06155	1147524..1148873	-	1350	WP_000886210.1	cell wall metabolism sensor histidine kinase VicK	-
EL271_RS06160	1148866..1149570	-	705	WP_000722076.1	response regulator transcription factor	-
EL271_RS06165	1149625..1150800	-	1176	WP_000886147.1	A/G-specific adenine glycosylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-84)

Antitoxin

• Protein of unknown function DUF6290 (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.85 Da
• Isoelectric Point: 10.7632

>T32473 WP_001812387.1 NZ_AP018937:c1146378-1146124 [Streptococcus pneumoniae]
MYKLVPTRRFIKQLKKLDRYTQKLITNYLQINVLEDPRRHGKALVGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHR
RDIY

Nucleotide

• Length: 255 bp

>T32473 NZ_AP018937:c1146378-1146124 [Streptococcus pneumoniae]
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAATCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTTGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9205.46 Da
• Isoelectric Point: 4.0527

>AT32473 WP_000208080.1 NZ_AP018937:c1146622-1146380 [Streptococcus pneumoniae]
MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYDARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE

Nucleotide

• Length: 243 bp

>AT32473 NZ_AP018937:c1146622-1146380 [Streptococcus pneumoniae]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA

Structures

Antitoxin

• AlphaFold DB: A0A064C2J7