Detailed information of TA system TA032472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 1078503..1079749
• Replicon: chromosome
• Accession: NZ_AP018937
• Organism: Streptococcus pneumoniae strain HU-OH

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: EL271_RS05805
• Protein ID: WP_000405354.1
• Coordinates: 1078979..1079749 (+)
• Length: 257 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: B6ZCC4
• Locus tag: EL271_RS05800
• Protein ID: WP_000579612.1
• Coordinates: 1078503..1078979 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL271_RS05765	1073693..1073941	+	249	WP_001814254.1	hypothetical protein	-
EL271_RS05770	1074112..1074831	+	720	Protein_1076	type A chloramphenicol O-acetyltransferase	-
EL271_RS05780	1075627..1076547	-	921	WP_029658194.1	protein rep	-
EL271_RS05790	1076674..1077504	+	831	Protein_1078	alpha-glycosidase	-
EL271_RS05795	1077772..1078227	+	456	WP_025173603.1	hypothetical protein	-
EL271_RS05800	1078503..1078979	+	477	WP_000579612.1	helix-turn-helix domain-containing protein	Antitoxin
EL271_RS05805	1078979..1079749	+	771	WP_000405354.1	zeta toxin family protein	Toxin
EL271_RS05810	1080005..1080688	+	684	WP_000492698.1	LexA family transcriptional regulator	-
EL271_RS05815	1080691..1082106	+	1416	WP_000118333.1	Y-family DNA polymerase	-
EL271_RS05820	1082103..1082468	+	366	WP_000981955.1	hypothetical protein	-
EL271_RS05825	1082458..1082748	+	291	WP_001080184.1	hypothetical protein	-
EL271_RS05830	1083043..1083399	+	357	WP_000827835.1	hypothetical protein	-
EL271_RS05835	1083404..1083769	+	366	WP_000401236.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B) / tet(M) / cat(pC194)	-	1036998..1092927	55929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 257 a.a.
• Molecular weight: 29234.14 Da
• Isoelectric Point: 7.5403

>T32472 WP_000405354.1 NZ_AP018937:1078979-1079749 [Streptococcus pneumoniae]
VEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTELSHLGYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVNNTRQLEELAIFERIQIYQRDRSCVYDSGEDKTSAATVLHDLLFGEWNQVEKEML
KSGEERLKDLTNRNGC

Nucleotide

• Length: 771 bp

>T32472 NZ_AP018937:1078979-1079749 [Streptococcus pneumoniae]
GTGGAAATCCAAGATTATACTGACAGTGAATTCAAACATGCTCTAGCACGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTCGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGAGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGATAGTGTAGAATACACCAAAGATTTTGCAGGTAAAATGGTAGAGTCTTTAGTAACAGAATTGAGTCA
TTTGGGATACAATCTTTTGATAGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTAGCCTTGATTGCGACAAAACCTAAGCTGTCCTATCTGAGCACCCTTATCCGATACGAAGAA
CTATACGCTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTAATAA
TACACGACAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAG
GAGAAGATAAAACTTCAGCAGCAACCGTTCTTCATGATTTACTATTTGGAGAATGGAATCAAGTGGAGAAAGAGATGCTT
AAATCTGGAGAAGAAAGATTGAAAGATTTAACTAATCGAAATGGTTGTTAG

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18245.68 Da
• Isoelectric Point: 4.4188

>AT32472 WP_000579612.1 NZ_AP018937:1078503-1078979 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT32472 NZ_AP018937:1078503-1078979 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACCGAATTAATAGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGACTATGAATTGACTTTAAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTTTTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGCGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAACGGATGGTAGCCTAG

Structures

Antitoxin

• AlphaFold DB: B6ZCC4