Detailed information of TA system TA032471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 1070956..1072190
• Replicon: chromosome
• Accession: NZ_AP018937
• Organism: Streptococcus pneumoniae strain HU-OH

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: B6ZCD1
• Locus tag: EL271_RS05755
• Protein ID: WP_000354294.1
• Coordinates: 1071432..1072190 (+)
• Length: 253 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: B6ZCD2
• Locus tag: EL271_RS05750
• Protein ID: WP_000579617.1
• Coordinates: 1070956..1071432 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL271_RS05710	1067239..1067523	+	285	WP_000969645.1	hypothetical protein	-
EL271_RS05715	1067537..1067832	+	296	Protein_1065	hypothetical protein	-
EL271_RS05720	1067846..1068043	-	198	WP_074187117.1	hypothetical protein	-
EL271_RS05725	1068178..1068819	+	642	WP_000933078.1	hypothetical protein	-
EL271_RS05730	1068829..1069914	+	1086	WP_000401904.1	toprim domain-containing protein	-
EL271_RS05735	1069965..1070198	+	234	WP_061746195.1	hypothetical protein	-
EL271_RS05740	1070195..1070578	+	384	WP_000159213.1	hypothetical protein	-
EL271_RS05745	1070616..1070888	+	273	WP_000495438.1	hypothetical protein	-
EL271_RS05750	1070956..1071432	+	477	WP_000579617.1	helix-turn-helix domain-containing protein	Antitoxin
EL271_RS05755	1071432..1072190	+	759	WP_000354294.1	zeta toxin family protein	Toxin
EL271_RS05760	1072666..1073454	+	789	WP_000214003.1	hypothetical protein	-
EL271_RS05765	1073693..1073941	+	249	WP_001814254.1	hypothetical protein	-
EL271_RS05770	1074112..1074831	+	720	Protein_1076	type A chloramphenicol O-acetyltransferase	-
EL271_RS05780	1075627..1076547	-	921	WP_029658194.1	protein rep	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B) / tet(M) / cat(pC194)	-	1036998..1092927	55929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 253 a.a.
• Molecular weight: 28929.87 Da
• Isoelectric Point: 7.5458

>T32471 WP_000354294.1 NZ_AP018937:1071432-1072190 [Streptococcus pneumoniae]
MDIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKYFAGKMVESLVRELSHLGYNLLIEGTLRTIDVPKKTAQLLKSRGYEIQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRGCVYDAKENTTSAADVLQELLFGEWSQVEKDML
KSGEERLKDLTN

Nucleotide

• Length: 759 bp

>T32471 NZ_AP018937:1071432-1072190 [Streptococcus pneumoniae]
ATGGATATCCAAGATTATACTGATAGTGAATTCAAACATGCTCTAGCACGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGAGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTTCAGCAA
GAATATGGCAAAGACAGTGTTGAATACACCAAATATTTTGCAGGTAAAATGGTAGAGTCTTTAGTAAGAGAATTGAGTCA
TTTGGGATACAATCTTTTGATAGAGGGAACTTTACGAACTATTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAGTA
GGGGATATGAAATACAATTAGCCTTGATTGCGACAAAGCCTAAGCTGTCCTATCTGAGCACCCTTATCCGATACGAAGAA
CTGTACGCTATTAACCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAGGTTGTGTATATGATGCAA
AAGAAAATACAACTTCAGCGGCAGATGTTCTTCAAGAGTTACTGTTTGGGGAGTGGAGTCAAGTAGAGAAGGATATGCTT
AAATCTGGAGAAGAAAGATTGAAAGATTTAACTAATTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18286.74 Da
• Isoelectric Point: 4.4977

>AT32471 WP_000579617.1 NZ_AP018937:1070956-1071432 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQPEFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDNRGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT32471 NZ_AP018937:1070956-1071432 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACCCAACCCGAATTTGCACGGATTGTAGGTATTTC
ACGTAATAGTCTGAGTCGATATGAAAATGGAACGAGTTCAGTTTCTACGGAACTAATAGACATCATTTGCCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTAACTTTAAAAATTGAA
ATTGTGAAAGAAAGAGGTGCGAATCTATTATCTCGACTCTATCGTTATCAAGATAATCGGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATTTATCTGATTTGATTCATACGAATATCTATCTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGCGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAACGGATGGTAGCCTAA

Structures

Toxin

• AlphaFold DB: B6ZCD1

Antitoxin

• AlphaFold DB: B6ZCD2