Detailed information of TA system TA032470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 910928..912165
• Replicon: chromosome
• Accession: NZ_AP018937
• Organism: Streptococcus pneumoniae strain HU-OH

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A4J1ZCF4
• Locus tag: EL271_RS04925
• Protein ID: WP_000405368.1
• Coordinates: 911404..912165 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: EL271_RS04920
• Protein ID: WP_000579602.1
• Coordinates: 910928..911404 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL271_RS04900	906001..906855	+	855	WP_000900019.1	ADP-ribosylglycohydrolase family protein	-
EL271_RS04905	906967..907851	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
EL271_RS04910	908014..909753	+	1740	WP_000423464.1	glycoside hydrolase family 13 protein	-
EL271_RS04915	910028..910483	+	456	WP_000500478.1	hypothetical protein	-
EL271_RS04920	910928..911404	+	477	WP_000579602.1	helix-turn-helix domain-containing protein	Antitoxin
EL271_RS04925	911404..912165	+	762	WP_000405368.1	zeta toxin family protein	Toxin
EL271_RS04930	912193..913350	+	1158	WP_001213951.1	hypothetical protein	-
EL271_RS04945	914182..914541	+	360	WP_000529545.1	hypothetical protein	-
EL271_RS04950	914551..914916	+	366	WP_000263039.1	MobC family plasmid mobilization relaxosome protein	-
EL271_RS11545	914903..916753	+	1851	Protein_920	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29185.27 Da
• Isoelectric Point: 7.5090

>T32470 WP_000405368.1 NZ_AP018937:911404-912165 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFVGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
RVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T32470 NZ_AP018937:911404-912165 [Streptococcus pneumoniae]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAACAACCTATAGCGATTTTGCTTGGAGGTCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGACAGTGTAGAATATACCAAAGATTTTGTAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGAGATACAATCTTTTGATCGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTAGCTATTTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTCTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
AGGGTAGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18289.70 Da
• Isoelectric Point: 4.4188

>AT32470 WP_000579602.1 NZ_AP018937:910928-911404 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQHEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT32470 NZ_AP018937:910928-911404 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACACGAATTTGCACGGATTATAGGTATTTC
ACGAAATAGTCTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACCGAATTAATAGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCAGACAAATATCTATTTAGTAGAAAATTTTGATG
AAATAGAGAGATATAGCGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A4J1ZCF4