Detailed information of TA system TA032469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1706940..1707602
• Replicon: chromosome
• Accession: NZ_AP018936
• Organism: Streptococcus pneumoniae strain NU83127

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EL277_RS08960
• Protein ID: WP_000192221.1
• Coordinates: 1707429..1707602 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: G0IC64
• Locus tag: EL277_RS08955
• Protein ID: WP_000961810.1
• Coordinates: 1706940..1707392 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL277_RS08930	1702830..1703573	-	744	WP_001188203.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
EL277_RS08935	1703575..1704525	-	951	WP_000451135.1	50S ribosomal protein L11 methyltransferase	-
EL277_RS08940	1704663..1705091	-	429	WP_000401988.1	NUDIX hydrolase	-
EL277_RS08945	1705093..1706160	-	1068	WP_061364929.1	M50 family metallopeptidase	-
EL277_RS08950	1706179..1706649	-	471	WP_000257084.1	DUF3013 family protein	-
EL277_RS08955	1706940..1707392	-	453	WP_000961810.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EL277_RS08960	1707429..1707602	-	174	WP_000192221.1	type II toxin-antitoxin system HicA family toxin	Toxin
EL277_RS08965	1707745..1707924	-	180	WP_001809903.1	hypothetical protein	-
EL277_RS08975	1708089..1708328	-	240	WP_000818078.1	hypothetical protein	-
EL277_RS08985	1708598..1709869	+	1272	WP_001113209.1	replication-associated recombination protein A	-
EL277_RS08995	1710288..1711415	-	1128	WP_126443150.1	site-specific integrase	-
EL277_RS09000	1711415..1711597	-	183	WP_061590400.1	DUF3173 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (17-114)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6368.47 Da
• Isoelectric Point: 10.8214

>T32469 WP_000192221.1 NZ_AP018936:c1707602-1707429 [Streptococcus pneumoniae]
MTQKEMVKLLTAHGWIKTRGGKGSHIKIEKQGERPITILHGELNKYTERGIGKQAGL

Nucleotide

• Length: 174 bp

>T32469 NZ_AP018936:c1707602-1707429 [Streptococcus pneumoniae]
ATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCATATTAA
AATCGAGAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAGGAAAGC
AAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16659.46 Da
• Isoelectric Point: 3.7220

>AT32469 WP_000961810.1 NZ_AP018936:c1707392-1706940 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Nucleotide

• Length: 453 bp

>AT32469 NZ_AP018936:c1707392-1706940 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Antitoxin

• AlphaFold DB: G0IC64