Detailed information of TA system TA032466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 995399..996636
• Replicon: chromosome
• Accession: NZ_AP018936
• Organism: Streptococcus pneumoniae strain NU83127

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: Q97QZ1
• Locus tag: EL277_RS05295
• Protein ID: WP_000405360.1
• Coordinates: 995875..996636 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: EL277_RS05290
• Protein ID: WP_000579608.1
• Coordinates: 995399..995875 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL277_RS05260	990472..991326	+	855	WP_000900015.1	ADP-ribosylglycohydrolase family protein	-
EL277_RS05265	991438..992322	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
EL277_RS05270	992485..992847	+	363	WP_000423476.1	alpha amylase N-terminal ig-like domain-containing protein	-
EL277_RS05275	992828..994224	+	1397	Protein_978	glycoside hydrolase family 13 protein	-
EL277_RS05280	994310..994627	+	318	WP_000995815.1	hypothetical protein	-
EL277_RS05285	994673..994954	+	282	WP_000266234.1	hypothetical protein	-
EL277_RS05290	995399..995875	+	477	WP_000579608.1	helix-turn-helix domain-containing protein	Antitoxin
EL277_RS05295	995875..996636	+	762	WP_000405360.1	toxin PezT	Toxin
EL277_RS05300	996664..997820	+	1157	Protein_983	hypothetical protein	-
EL277_RS05310	998652..999008	+	357	WP_000817623.1	hypothetical protein	-
EL277_RS05315	999013..999378	+	366	WP_000401239.1	MobC family plasmid mobilization relaxosome protein	-
EL277_RS05320	999365..1001188	+	1824	WP_000237144.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	971025..1113420	142395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29129.20 Da
• Isoelectric Point: 7.5087

>T32466 WP_000405360.1 NZ_AP018936:995875-996636 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T32466 NZ_AP018936:995875-996636 [Streptococcus pneumoniae]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGACAGTGTAGAATATACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGAGATACAATCTTTTGATAGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
CACACGAAAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTCTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
CAGGTGGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18247.70 Da
• Isoelectric Point: 4.4188

>AT32466 WP_000579608.1 NZ_AP018936:995399-995875 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQLEFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT32466 NZ_AP018936:995399-995875 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTGCGTAAAACACATGACTTAACACAACTCGAATTTGCACGGATTGTAGGTATTTC
ACGAAATAGTCTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACCGAATTAATAGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTAAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
GTCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGATTTGATTCATACGAATATCTATCTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAACGGATGGTGGCCTAA

Structures

Toxin

• PDB: 2P5T