Detailed information of TA system TA032461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1324248..1324870
• Replicon: chromosome
• Accession: NZ_AP018933
• Organism: Zymobacter palmae strain IAM14233

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ZBT109_RS05845
• Protein ID: WP_027704792.1
• Coordinates: 1324248..1324430 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A348HED1
• Locus tag: ZBT109_RS05850
• Protein ID: WP_027704791.1
• Coordinates: 1324463..1324870 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ZBT109_RS05815	1319514..1319927	-	414	WP_027704795.1	hypothetical protein	-
ZBT109_RS05820	1319930..1321582	-	1653	WP_027704794.1	phage terminase large subunit	-
ZBT109_RS05825	1321575..1322561	-	987	WP_051523722.1	terminase small subunit	-
ZBT109_RS05830	1322577..1322753	-	177	WP_084261757.1	ogr/Delta-like zinc finger family protein	-
ZBT109_RS05835	1323039..1323407	+	369	WP_027704793.1	hypothetical protein	-
ZBT109_RS05840	1323443..1323988	-	546	WP_084261756.1	KilA-N domain-containing protein	-
ZBT109_RS05845	1324248..1324430	+	183	WP_027704792.1	type II toxin-antitoxin system HicA family toxin	Toxin
ZBT109_RS05850	1324463..1324870	+	408	WP_027704791.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ZBT109_RS05855	1324938..1326344	-	1407	WP_027704790.1	glycoside hydrolase family 68 protein	-
ZBT109_RS05860	1326428..1326781	-	354	WP_051523721.1	phage holin, lambda family	-
ZBT109_RS05865	1327051..1327491	-	441	WP_051523720.1	hypothetical protein	-
ZBT109_RS05870	1327496..1327936	-	441	WP_084261754.1	L,D-transpeptidase	-
ZBT109_RS05875	1328029..1328721	-	693	WP_027704789.1	hypothetical protein	-
ZBT109_RS05880	1328718..1329095	-	378	WP_051523719.1	hypothetical protein	-
ZBT109_RS05885	1329092..1329412	-	321	WP_027704788.1	DUF1364 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1302318..1348153	45835

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6723.86 Da
• Isoelectric Point: 10.8183

>T32461 WP_027704792.1 NZ_AP018933:1324248-1324430 [Zymobacter palmae]
VKSSDVIKELEKDGWYCVRIKGSHHHFRHPSKPGTVTVPHPKKELQIGLVRAIRKSAGLA

Nucleotide

• Length: 183 bp

>T32461 NZ_AP018933:1324248-1324430 [Zymobacter palmae]
GTGAAAAGCAGTGATGTGATCAAAGAGCTTGAAAAAGATGGCTGGTACTGTGTTCGAATCAAAGGCAGCCACCATCACTT
CAGGCACCCAAGCAAGCCCGGCACAGTGACCGTGCCCCATCCTAAAAAGGAATTGCAGATAGGTCTTGTTAGGGCGATTA
GAAAAAGCGCCGGCCTCGCATGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14894.72 Da
• Isoelectric Point: 5.2347

>AT32461 WP_027704791.1 NZ_AP018933:1324463-1324870 [Zymobacter palmae]
MLYPIAIERGDEKHAHSVAVPDIPGCFSAGDSFDDALRNAKEAIEGHLETLAEFGDDIPRASAVDAHCDNPAYKGWVWAV
VDIDISRYLGKSQKINVSLPERVIRRIDDTVAKNAAYRTRSGFLAQAALRELERS

Nucleotide

• Length: 408 bp

>AT32461 NZ_AP018933:1324463-1324870 [Zymobacter palmae]
ATGCTGTATCCCATTGCAATTGAGCGTGGAGACGAAAAGCATGCACATAGCGTTGCCGTTCCCGATATACCCGGCTGCTT
CTCTGCGGGTGACTCGTTTGATGACGCTCTACGCAACGCCAAGGAAGCCATCGAAGGGCACTTGGAAACGTTGGCTGAGT
TTGGTGATGACATCCCACGGGCAAGCGCTGTCGATGCTCACTGCGATAATCCGGCCTACAAAGGCTGGGTATGGGCTGTA
GTGGACATTGATATATCGCGTTACCTCGGCAAAAGCCAAAAAATCAACGTCTCACTGCCCGAACGTGTTATTCGTCGCAT
TGATGACACGGTGGCCAAGAACGCTGCCTATAGAACGCGATCTGGTTTTCTAGCTCAAGCTGCTTTGCGTGAGTTAGAAC
GCTCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A348HED1