Detailed information of TA system TA032458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/NGN_SP-AbrB
• Location: 527515..528106
• Replicon: chromosome
• Accession: NZ_AP018930
• Organism: Sulfuracidifex tepidarius strain IC-007

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F1551_RS02455
• Protein ID: WP_232048982.1
• Coordinates: 527729..528106 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A510DT36
• Locus tag: F1551_RS02450
• Protein ID: WP_054846032.1
• Coordinates: 527515..527748 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1551_RS02410 (IC007_0556)	522557..523069	+	513	WP_054846038.1	MFS transporter	-
F1551_RS02415 (IC007_0557)	523396..523830	+	435	WP_232516047.1	HEPN domain-containing protein	-
F1551_RS02420 (IC007_0558)	523880..524155	+	276	WP_269777893.1	nucleotidyltransferase domain-containing protein	-
F1551_RS02425 (IC007_0559)	524857..525099	+	243	WP_054846070.1	hypothetical protein	-
F1551_RS02430 (IC007_0560)	525089..525481	+	393	WP_054846035.1	type II toxin-antitoxin system VapC family toxin	-
F1551_RS02435	525576..525767	+	192	WP_149528309.1	hypothetical protein	-
F1551_RS02440 (IC007_0561)	526070..526483	-	414	WP_054846034.1	PIN domain-containing protein	-
F1551_RS02445 (IC007_0562)	526455..526703	-	249	WP_054846033.1	hypothetical protein	-
F1551_RS13595	526801..526920	+	120	Protein_516	IS5/IS1182 family transposase	-
F1551_RS02450 (IC007_0563)	527515..527748	+	234	WP_054846032.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
F1551_RS02455 (IC007_0564)	527729..528106	+	378	WP_232048982.1	type II toxin-antitoxin system VapC family toxin	Toxin
F1551_RS02460 (IC007_0566)	528973..529152	+	180	WP_054846030.1	hypothetical protein	-
F1551_RS02465 (IC007_0568)	529715..530026	-	312	WP_269777886.1	type II toxin-antitoxin system VapC family toxin	-
F1551_RS13070	531122..531265	-	144	WP_162302101.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-116)

Antitoxin

• SpoVT-AbrB domain (5-43)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14208.60 Da
• Isoelectric Point: 9.6828

>T32458 WP_232048982.1 NZ_AP018930:527729-528106 [Sulfuracidifex tepidarius]
VRKVMLDASAIMLYFYGNEKLKEIIENSEVYVNSVNLTEFLYAYARAKGWKEGLFKYSIIRNSFKLIDVNKDDIITTSAK
LKIRYNLSLGDSFLLASAKHIKGVAVTSDHELKDVKEVKVLVVSL

Nucleotide

• Length: 378 bp

>T32458 NZ_AP018930:527729-528106 [Sulfuracidifex tepidarius]
GTGAGAAAAGTAATGCTTGACGCATCGGCAATTATGCTATATTTCTATGGGAATGAGAAACTGAAGGAGATAATAGAAAA
TAGTGAAGTTTACGTTAATTCTGTAAATCTCACAGAGTTCCTTTATGCTTACGCCAGAGCTAAAGGATGGAAAGAAGGAC
TATTCAAATACAGTATCATAAGAAACTCCTTTAAACTGATTGATGTAAATAAAGATGATATAATTACTACTTCTGCTAAG
TTGAAAATTAGGTATAACCTTTCTCTGGGGGACTCTTTCCTTTTAGCATCAGCTAAACATATTAAAGGAGTTGCAGTAAC
TAGCGACCATGAGCTCAAGGACGTAAAAGAGGTAAAAGTTCTAGTAGTTTCTTTGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 9026.47 Da
• Isoelectric Point: 9.4248

>AT32458 WP_054846032.1 NZ_AP018930:527515-527748 [Sulfuracidifex tepidarius]
MYRLKVHKKGITVIPKEIRDKFNIKEGDEVNVIVDDKGIYIFPMQTIEKYFGSDKGAIDALKILEEERRKEREKSNA

Nucleotide

• Length: 234 bp

>AT32458 NZ_AP018930:527515-527748 [Sulfuracidifex tepidarius]
ATGTATAGGTTAAAAGTTCATAAAAAGGGGATTACGGTAATACCCAAAGAGATTAGAGATAAGTTCAACATAAAAGAGGG
TGACGAGGTAAACGTTATAGTCGACGATAAAGGTATTTATATATTTCCTATGCAGACTATTGAAAAATATTTCGGAAGCG
ATAAAGGTGCTATAGATGCTCTAAAAATTTTAGAGGAAGAGAGGAGAAAAGAACGTGAGAAAAGTAATGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A510DT36