Detailed information of TA system TA032448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2193194..2193723
• Replicon: chromosome
• Accession: NZ_AP018923
• Organism: Staphylococcus aureus strain JMUB3031

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D5070_RS11480
• Protein ID: WP_000621175.1
• Coordinates: 2193194..2193556 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: D5070_RS11485
• Protein ID: WP_000948331.1
• Coordinates: 2193553..2193723 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D5070_RS11455	2190172..2190942	-	771	WP_117201032.1	RNA polymerase sigma factor SigB	-
D5070_RS11460	2190917..2191396	-	480	WP_117201034.1	anti-sigma B factor RsbW	-
D5070_RS11465	2191398..2191724	-	327	WP_001052491.1	anti-sigma factor antagonist	-
D5070_RS11470	2191843..2192844	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
D5070_RS11480	2193194..2193556	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D5070_RS11485	2193553..2193723	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
D5070_RS11490	2193808..2194956	-	1149	WP_001281154.1	alanine racemase	-
D5070_RS11495	2195022..2195381	-	360	WP_000581197.1	holo-ACP synthase	-
D5070_RS11500	2195385..2195876	-	492	WP_001205912.1	PH domain-containing protein	-
D5070_RS11505	2195863..2197446	-	1584	WP_001294626.1	PH domain-containing protein	-
D5070_RS11510	2197439..2197918	-	480	WP_001287078.1	hypothetical protein	-
D5070_RS11515	2198127..2198687	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T32448 WP_000621175.1 NZ_AP018923:c2193556-2193194 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T32448 NZ_AP018923:c2193556-2193194 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT32448 WP_000948331.1 NZ_AP018923:c2193723-2193553 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT32448 NZ_AP018923:c2193723-2193553 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1