Detailed information of TA system TA032428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2067563..2068092
• Replicon: chromosome
• Accession: NZ_AP018922
• Organism: Staphylococcus aureus strain JMUB1273

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JMUB1273_RS10640
• Protein ID: WP_000621175.1
• Coordinates: 2067563..2067925 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JMUB1273_RS10645
• Protein ID: WP_000948331.1
• Coordinates: 2067922..2068092 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMUB1273_RS10615	2064541..2065311	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
JMUB1273_RS10620	2065286..2065765	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
JMUB1273_RS10625	2065767..2066093	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JMUB1273_RS10630	2066212..2067213	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JMUB1273_RS10640	2067563..2067925	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JMUB1273_RS10645	2067922..2068092	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JMUB1273_RS10650	2068177..2069325	-	1149	WP_001281154.1	alanine racemase	-
JMUB1273_RS10655	2069391..2069750	-	360	WP_000581197.1	holo-ACP synthase	-
JMUB1273_RS10660	2069754..2070245	-	492	WP_001205912.1	PH domain-containing protein	-
JMUB1273_RS10665	2070232..2071815	-	1584	WP_001294620.1	PH domain-containing protein	-
JMUB1273_RS10670	2071808..2072287	-	480	WP_001287079.1	hypothetical protein	-
JMUB1273_RS10675	2072496..2073056	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T32428 WP_000621175.1 NZ_AP018922:c2067925-2067563 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T32428 NZ_AP018922:c2067925-2067563 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT32428 WP_000948331.1 NZ_AP018922:c2068092-2067922 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT32428 NZ_AP018922:c2068092-2067922 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1