Detailed information of TA system TA032406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 8483..9071
• Replicon: plasmid pAURM-2
• Accession: NZ_AP018826
• Organism: Acinetobacter ursingii strain M3

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: URS_RS16680
• Protein ID: WP_126624547.1
• Coordinates: 8483..8770 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: URS_RS16685
• Protein ID: WP_126624548.1
• Coordinates: 8757..9071 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
URS_RS16645	3881..4072	-	192	WP_017482457.1	zf-HC2 domain-containing protein	-
URS_RS16650	4066..4683	-	618	WP_126624542.1	RNA polymerase factor sigma-70	-
URS_RS16655	4693..5436	-	744	WP_125280766.1	putative DNA-binding domain-containing protein	-
URS_RS16660	5472..6299	-	828	WP_126624543.1	DUF692 domain-containing protein	-
URS_RS16665	6380..6721	-	342	WP_126624544.1	hypothetical protein	-
URS_RS16670	6897..7511	+	615	WP_126624545.1	DoxX family protein	-
URS_RS16675	7724..8116	-	393	WP_126624546.1	hypothetical protein	-
URS_RS16680	8483..8770	+	288	WP_126624547.1	BrnT family toxin	Toxin
URS_RS16685	8757..9071	+	315	WP_126624548.1	BrnA antitoxin family protein	Antitoxin
URS_RS16695	10765..12381	+	1617	WP_197723802.1	histone H1-like repetitive region-containing protein	-
URS_RS16700	12471..13871	+	1401	WP_057692806.1	DUF560 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33916	33916

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11248.59 Da
• Isoelectric Point: 5.2063

>T32406 WP_126624547.1 NZ_AP018826:8483-8770 [Acinetobacter ursingii]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTVGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNQYEHG

Nucleotide

• Length: 288 bp

>T32406 NZ_AP018826:8483-8770 [Acinetobacter ursingii]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAACACGATGTCTCTTTCGAAACAGCAAG
CCTTGTTTTTGAAGATCCTCTAAGGATCTCAATCCAGGACAGACATACCGATGGTGAAGAACGTTGGCAAACCGTTGGAA
AAGTAAAAGGCGTACTAATGCTGTTAGTAGCTCACACTATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTCACCAAAGCGGAGCGAAATCAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12299.88 Da
• Isoelectric Point: 6.4741

>AT32406 WP_126624548.1 NZ_AP018826:8757-9071 [Acinetobacter ursingii]
MSMVRYTRKELNENFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILREAMLEDLKNHR

Nucleotide

• Length: 315 bp

>AT32406 NZ_AP018826:8757-9071 [Acinetobacter ursingii]
ATGAGCATGGTTAGATATACACGCAAAGAGCTGAATGAAAACTTCAGCGACAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAGGGAACGGTTCCTGACGATCAGCTAGATCTCTCAGATATGCCTGAAATTACCGACTGGAGCAATGCTGTACGCC
ATAATCAATTCTATCGCCCAGTGAAGCAACAGACTTCCATTCGTTTAGATGCTGATGTACTTGCATGGTTTAAAGCTCAA
GGCAAAGGCTATCAAACACGAATGAATGAAATTCTGCGTGAAGCAATGTTAGAAGATTTAAAAAATCATCGATAA