Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tad-ata/Tad-HTH_37 |
| Location | 14308..15005 | Replicon | plasmid pBAR1 |
| Accession | NZ_AP018819 | ||
| Organism | Sphingomonas bisphenolicum strain AO1 | ||
Toxin (Protein)
| Gene name | tad | Uniprot ID | T0J3S2 |
| Locus tag | SBA_RS22710 | Protein ID | WP_013041565.1 |
| Coordinates | 14631..15005 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | ata | Uniprot ID | T0G937 |
| Locus tag | SBA_RS22705 | Protein ID | WP_006964370.1 |
| Coordinates | 14308..14634 (-) | Length | 109 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SBA_RS22675 (SBA_pBAR1_100) | 9965..10720 | + | 756 | WP_006964381.1 | P-type conjugative transfer protein TrbJ | - |
| SBA_RS22680 (SBA_pBAR1_110) | 10730..10912 | + | 183 | WP_006964379.1 | hypothetical protein | - |
| SBA_RS22685 (SBA_pBAR1_120) | 10923..12638 | + | 1716 | WP_006964377.1 | P-type conjugative transfer protein TrbL | - |
| SBA_RS22690 (SBA_pBAR1_130) | 12641..13753 | + | 1113 | WP_006964375.1 | IncP-type conjugative transfer protein TrbN | - |
| SBA_RS22695 (SBA_pBAR1_140) | 13774..14073 | - | 300 | WP_006964373.1 | hypothetical protein | - |
| SBA_RS22700 (SBA_pBAR1_150) | 14066..14308 | - | 243 | WP_006964372.1 | hypothetical protein | - |
| SBA_RS22705 (SBA_pBAR1_160) | 14308..14634 | - | 327 | WP_006964370.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| SBA_RS22710 (SBA_pBAR1_170) | 14631..15005 | - | 375 | WP_013041565.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| SBA_RS22715 (SBA_pBAR1_180) | 15261..15608 | - | 348 | Protein_18 | IS6-like element IS6100 family transposase | - |
| SBA_RS22720 (SBA_pBAR1_190) | 15672..16432 | + | 761 | Protein_19 | IS6-like element IS6100 family transposase | - |
| SBA_RS22725 (SBA_pBAR1_200) | 16534..17805 | - | 1272 | WP_261937507.1 | cytochrome P450 | - |
| SBA_RS22730 (SBA_pBAR1_210) | 17880..18197 | - | 318 | WP_022684651.1 | 2Fe-2S iron-sulfur cluster-binding protein | - |
| SBA_RS22735 (SBA_pBAR1_220) | 18544..19308 | - | 765 | WP_001389365.1 | IS6-like element IS6100 family transposase | - |
| SBA_RS22740 (SBA_pBAR1_230) | 19351..19650 | - | 300 | Protein_23 | P-type conjugative transfer ATPase TrbB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..80312 | 80312 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 13960.67 Da Isoelectric Point: 9.4885
>T32403 WP_013041565.1 NZ_AP018819:c15005-14631 [Sphingomonas bisphenolicum]
MDTERPVRWVASSKRDFREFPDDVQDVMGYALHLAQQGGQHASTKPLKGFGGAGVVEIIDDHQGDTFRTVYTVKFAEAVY
VLHAFQKKSKQGKATPQADMDLIRTRLKSAEEHHRQHQTPRGTA
MDTERPVRWVASSKRDFREFPDDVQDVMGYALHLAQQGGQHASTKPLKGFGGAGVVEIIDDHQGDTFRTVYTVKFAEAVY
VLHAFQKKSKQGKATPQADMDLIRTRLKSAEEHHRQHQTPRGTA
Download Length: 375 bp
>T32403 NZ_AP018819:c15005-14631 [Sphingomonas bisphenolicum]
ATGGATACGGAACGGCCGGTTCGATGGGTGGCATCGTCAAAGCGCGATTTTCGGGAGTTCCCGGACGATGTGCAGGACGT
AATGGGCTATGCCCTGCACCTTGCGCAGCAAGGCGGGCAGCACGCCAGCACTAAGCCGTTGAAGGGCTTTGGCGGCGCTG
GCGTGGTCGAGATCATAGACGACCACCAAGGCGATACGTTCCGCACGGTCTATACGGTCAAATTCGCGGAGGCGGTCTAT
GTCCTGCACGCCTTCCAGAAGAAATCCAAGCAGGGCAAGGCCACGCCGCAGGCCGATATGGATTTGATCCGAACGCGGCT
GAAATCCGCCGAGGAGCATCACCGGCAACACCAGACCCCGCGAGGCACAGCATGA
ATGGATACGGAACGGCCGGTTCGATGGGTGGCATCGTCAAAGCGCGATTTTCGGGAGTTCCCGGACGATGTGCAGGACGT
AATGGGCTATGCCCTGCACCTTGCGCAGCAAGGCGGGCAGCACGCCAGCACTAAGCCGTTGAAGGGCTTTGGCGGCGCTG
GCGTGGTCGAGATCATAGACGACCACCAAGGCGATACGTTCCGCACGGTCTATACGGTCAAATTCGCGGAGGCGGTCTAT
GTCCTGCACGCCTTCCAGAAGAAATCCAAGCAGGGCAAGGCCACGCCGCAGGCCGATATGGATTTGATCCGAACGCGGCT
GAAATCCGCCGAGGAGCATCACCGGCAACACCAGACCCCGCGAGGCACAGCATGA
Antitoxin
Download Length: 109 a.a. Molecular weight: 11792.57 Da Isoelectric Point: 7.1681
>AT32403 WP_006964370.1 NZ_AP018819:c14634-14308 [Sphingomonas bisphenolicum]
MSAQDEIIEEGSGNVFADLGFADPDTHLLKAQLVTRVAEAMQERKLTQIAAAKVTGSTQPELSRILRGQFRSVSVERLLA
MLTRLGCKVDIVVRPQGRTTESAVIHFA
MSAQDEIIEEGSGNVFADLGFADPDTHLLKAQLVTRVAEAMQERKLTQIAAAKVTGSTQPELSRILRGQFRSVSVERLLA
MLTRLGCKVDIVVRPQGRTTESAVIHFA
Download Length: 327 bp
>AT32403 NZ_AP018819:c14634-14308 [Sphingomonas bisphenolicum]
ATGAGCGCACAGGACGAAATCATTGAGGAAGGCAGCGGCAATGTCTTTGCCGATCTAGGCTTTGCCGACCCCGACACCCA
CCTTTTGAAAGCCCAGCTCGTTACCCGTGTAGCCGAGGCCATGCAGGAACGTAAATTGACGCAGATCGCCGCCGCCAAGG
TTACGGGATCTACCCAACCGGAATTGTCGCGCATCCTGCGAGGCCAGTTCCGCAGCGTCTCGGTTGAGCGGCTTCTGGCC
ATGCTCACCCGGCTTGGCTGTAAGGTCGATATTGTCGTGCGTCCGCAGGGGCGGACCACTGAATCGGCCGTGATCCACTT
CGCCTAA
ATGAGCGCACAGGACGAAATCATTGAGGAAGGCAGCGGCAATGTCTTTGCCGATCTAGGCTTTGCCGACCCCGACACCCA
CCTTTTGAAAGCCCAGCTCGTTACCCGTGTAGCCGAGGCCATGCAGGAACGTAAATTGACGCAGATCGCCGCCGCCAAGG
TTACGGGATCTACCCAACCGGAATTGTCGCGCATCCTGCGAGGCCAGTTCCGCAGCGTCTCGGTTGAGCGGCTTCTGGCC
ATGCTCACCCGGCTTGGCTGTAAGGTCGATATTGTCGTGCGTCCGCAGGGGCGGACCACTGAATCGGCCGTGATCCACTT
CGCCTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0J7XHG4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0J8A803 |