Detailed information of TA system TA032400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 53975..54497
• Replicon: plasmid pE2865-4
• Accession: NZ_AP018812
• Organism: Escherichia coli strain E2865

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: EL389_RS31720
• Protein ID: WP_000638823.1
• Coordinates: 53975..54256 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: EL389_RS31725
• Protein ID: WP_000121741.1
• Coordinates: 54246..54497 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL389_RS31685	50124..50444	-	321	WP_000362080.1	VirB3 family type IV secretion system protein	-
EL389_RS31690	50515..50703	-	189	WP_050567133.1	hypothetical protein	-
EL389_RS31695	50805..51389	-	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
EL389_RS31700	51410..51808	-	399	WP_001153665.1	hypothetical protein	-
EL389_RS31705	51927..52364	-	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
EL389_RS31710	52370..53605	-	1236	WP_015059538.1	TcpQ domain-containing protein	-
EL389_RS31715	53608..53907	-	300	WP_000835764.1	IncN plasmid KikA protein	-
EL389_RS31720	53975..54256	-	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EL389_RS31725	54246..54497	-	252	WP_000121741.1	hypothetical protein	Antitoxin
EL389_RS31730	54597..55232	-	636	WP_015059536.1	hypothetical protein	-
EL389_RS31735	55305..55592	-	288	WP_001032611.1	EexN family lipoprotein	-
EL389_RS31740	55605..55859	-	255	WP_001043555.1	EexN family lipoprotein	-
EL389_RS31745	55861..56502	-	642	WP_001425343.1	conjugal transfer protein TrbJ	-
EL389_RS31750	56508..57503	-	996	WP_001028543.1	type IV secretion system protein	-
EL389_RS31755	57507..57764	-	258	WP_000739144.1	hypothetical protein	-
EL389_RS31760	57761..58063	-	303	WP_001360345.1	hypothetical protein	-
EL389_RS31765	58044..58301	-	258	WP_001542015.1	hypothetical protein	-
EL389_RS31770	58334..58780	-	447	WP_001243165.1	hypothetical protein	-
EL389_RS32100	58791..58961	-	171	WP_000550720.1	hypothetical protein	-
EL389_RS31775	58965..59408	-	444	WP_000964330.1	NfeD family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..62235	62235

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T32400 WP_000638823.1 NZ_AP018812:c54256-53975 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T32400 NZ_AP018812:c54256-53975 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT32400 WP_000121741.1 NZ_AP018812:c54497-54246 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT32400 NZ_AP018812:c54497-54246 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1