Detailed information of TA system TA032399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 9214..9715
• Replicon: plasmid pE2865-4
• Accession: NZ_AP018812
• Organism: Escherichia coli strain E2865

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: EL389_RS31450
• Protein ID: WP_000702247.1
• Coordinates: 9214..9474 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: EL389_RS31455
• Protein ID: WP_001132407.1
• Coordinates: 9464..9715 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL389_RS31405	4811..4972	+	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	-
EL389_RS31410	5122..5718	+	597	WP_000125165.1	hypothetical protein	-
EL389_RS32215	5736..6020	-	285	WP_001162371.1	hypothetical protein	-
EL389_RS31420	6094..6759	-	666	WP_000532148.1	P-loop NTPase	-
EL389_RS31430	7357..7686	+	330	WP_000855120.1	hypothetical protein	-
EL389_RS31435	8268..8591	+	324	WP_000427403.1	hypothetical protein	-
EL389_RS31440	8632..8841	+	210	WP_000866036.1	hypothetical protein	-
EL389_RS31445	8938..9162	+	225	WP_000814381.1	hypothetical protein	-
EL389_RS31450	9214..9474	+	261	WP_000702247.1	hypothetical protein	Toxin
EL389_RS31455	9464..9715	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
EL389_RS31470	10264..10542	+	279	WP_000545938.1	hypothetical protein	-
EL389_RS31475	10962..11228	+	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
EL389_RS31480	11335..11517	-	183	WP_001481829.1	hypothetical protein	-
EL389_RS31485	12075..12257	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
EL389_RS31490	12282..12719	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
EL389_RS31495	12850..13176	-	327	WP_063078684.1	hypothetical protein	-
EL389_RS31500	13196..13903	-	708	WP_021503228.1	hypothetical protein	-
EL389_RS31505	13905..14657	-	753	WP_063078685.1	conjugal transfer protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..62235	62235

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T32399 WP_000702247.1 NZ_AP018812:9214-9474 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T32399 NZ_AP018812:9214-9474 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT32399 WP_001132407.1 NZ_AP018812:9464-9715 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT32399 NZ_AP018812:9464-9715 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0