Detailed information of TA system TA032398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1773..2203
• Replicon: plasmid pE2865-3
• Accession: NZ_AP018811
• Organism: Escherichia coli strain E2865

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: EL389_RS30785
• Protein ID: WP_001388374.1
• Coordinates: 2039..2203 (+)
• Length: 55 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A6Y0EDY2
• Locus tag: EL389_RS30780
• Protein ID: WP_000079939.1
• Coordinates: 1773..2042 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL389_RS30765	1..858	+	858	WP_126347167.1	incFII family plasmid replication initiator RepA	-
EL389_RS30780	1773..2042	+	270	WP_000079939.1	hypothetical protein	Antitoxin
EL389_RS30785	2039..2203	+	165	WP_001388374.1	hypothetical protein	Toxin
EL389_RS30790	2251..3174	+	924	WP_001415483.1	hypothetical protein	-
EL389_RS30800	3616..3996	+	381	WP_001696636.1	HTH domain-containing protein	-
EL389_RS30805	4108..4512	+	405	Protein_6	IS91 family transposase	-
EL389_RS30810	4619..5455	-	837	WP_000480972.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
EL389_RS30815	5455..6258	-	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
EL389_RS30820	6270..6518	-	249	Protein_9	Hin recombinase	-
EL389_RS30825	6518..6655	+	138	Protein_10	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / tet(A) / sul2	-	1..75799	75799

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 55 a.a.
• Molecular weight: 6604.73 Da
• Isoelectric Point: 7.1989

>T32398 WP_001388374.1 NZ_AP018811:2039-2203 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYFGRTHRLTPVLHLYYM

Nucleotide

• Length: 165 bp

>T32398 NZ_AP018811:2039-2203 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAATACATTGCGGAGCAGAACCTGAT
AGCAGCCATTGAACTGGACGAACGGATTGGTTATTTCGGACGAACGCACCGGCTTACGCCGGTTTTACATCTGTACTACA
TGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10233.54 Da
• Isoelectric Point: 6.7263

>AT32398 WP_000079939.1 NZ_AP018811:1773-2042 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT32398 NZ_AP018811:1773-2042 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCTGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGTCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCACGGCGATGTACTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGAGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6Y0EDY2