Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 91070..91671 Replicon plasmid pE2865-1
Accession NZ_AP018809
Organism Escherichia coli strain E2865

Toxin (Protein)


Gene name doc Uniprot ID U9Z1Q0
Locus tag EL389_RS30150 Protein ID WP_001216047.1
Coordinates 91291..91671 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag EL389_RS30145 Protein ID WP_001190712.1
Coordinates 91070..91291 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL389_RS30085 86204..86392 + 189 WP_000797279.1 hypothetical protein -
EL389_RS30090 86394..86909 + 516 WP_001229203.1 hypothetical protein -
EL389_RS30095 86906..87796 + 891 WP_064579285.1 DUF551 domain-containing protein -
EL389_RS30105 87879..88112 + 234 WP_032346477.1 hypothetical protein -
EL389_RS30110 88291..88584 + 294 WP_000269004.1 hypothetical protein -
EL389_RS30115 88591..88965 + 375 WP_032346478.1 hypothetical protein -
EL389_RS30120 88947..89597 + 651 WP_000057451.1 hypothetical protein -
EL389_RS30125 89581..89865 + 285 WP_001142394.1 hypothetical protein -
EL389_RS30130 89850..90059 - 210 WP_001344848.1 hypothetical protein -
EL389_RS30135 90233..90484 + 252 WP_001283837.1 DNA polymerase III subunit theta -
EL389_RS30140 90608..90997 + 390 WP_000506726.1 DNA repair protein -
EL389_RS30145 91070..91291 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EL389_RS30150 91291..91671 + 381 WP_001216047.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
EL389_RS30155 91676..91855 + 180 WP_000113019.1 hypothetical protein -
EL389_RS30160 91883..92926 + 1044 WP_000644102.1 DUF968 domain-containing protein -
EL389_RS30165 93015..93467 + 453 WP_001312282.1 late promoter-activating protein (Gp10) -
EL389_RS30170 93554..94747 + 1194 WP_024222339.1 hypothetical protein -
EL389_RS30175 94747..96240 + 1494 WP_024222340.1 terminase -
EL389_RS32180 96332..96562 + 231 Protein_106 ash family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..99498 99498


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13574.27 Da        Isoelectric Point: 5.1514

>T32396 WP_001216047.1 NZ_AP018809:91291-91671 [Escherichia coli]
MRHISPEELVALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T32396 NZ_AP018809:91291-91671 [Escherichia coli]
ATGAGACATATATCACCGGAAGAACTTGTTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT32396 WP_001190712.1 NZ_AP018809:91070-91291 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT32396 NZ_AP018809:91070-91291 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9Z1Q0

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References