Detailed information of TA system TA032393

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5562633..5563465
• Replicon: chromosome
• Accession: NZ_AP018808
• Organism: Escherichia coli strain E2865

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: EL389_RS28985
• Protein ID: WP_064579232.1
• Coordinates: 5563091..5563465 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: EL389_RS28980
• Protein ID: WP_001315620.1
• Coordinates: 5562633..5563001 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL389_RS28945	5559469..5559924	+	456	WP_000581504.1	hypothetical protein	-
EL389_RS28950	5560003..5560236	+	234	WP_001119729.1	DUF905 family protein	-
EL389_RS28960	5560336..5561154	+	819	WP_001234622.1	DUF945 domain-containing protein	-
EL389_RS28965	5561209..5561694	+	486	WP_000849588.1	antirestriction protein	-
EL389_RS28970	5561710..5562186	+	477	WP_001186774.1	RadC family protein	-
EL389_RS28975	5562249..5562470	+	222	WP_000692345.1	DUF987 domain-containing protein	-
EL389_RS28980	5562633..5563001	+	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EL389_RS28985	5563091..5563465	+	375	WP_064579232.1	TA system toxin CbtA family protein	Toxin
EL389_RS28990	5563462..5563950	+	489	WP_000777547.1	hypothetical protein	-
EL389_RS28995	5563962..5564159	+	198	WP_000839281.1	DUF957 domain-containing protein	-
EL389_RS32040	5564244..5564396	+	153	WP_001280445.1	hypothetical protein	-
EL389_RS29020	5565441..5566091	+	651	WP_001037966.1	HNH endonuclease	-
EL389_RS29025	5566099..5567079	-	981	WP_000991415.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
EL389_RS29030	5567144..5568250	-	1107	WP_001341302.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14021.05 Da
• Isoelectric Point: 8.2905

>T32393 WP_064579232.1 NZ_AP018808:5563091-5563465 [Escherichia coli]
MKTLPDTHVREASSCPSLVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T32393 NZ_AP018808:5563091-5563465 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCTCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT32393 WP_001315620.1 NZ_AP018808:5562633-5563001 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT32393 NZ_AP018808:5562633-5563001 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: V0ULY5