Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2518742..2519213 Replicon chromosome
Accession NZ_AP018808
Organism Escherichia coli strain E2865

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A1Y2Y8G6
Locus tag EL389_RS13590 Protein ID WP_001448501.1
Coordinates 2518935..2519213 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag EL389_RS13585 Protein ID WP_001302048.1
Coordinates 2518742..2518933 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL389_RS13535 2513820..2514038 - 219 WP_000683609.1 DUF4014 family protein -
EL389_RS13540 2514166..2514477 - 312 WP_001289673.1 hypothetical protein -
EL389_RS13545 2514470..2514697 - 228 WP_000699809.1 hypothetical protein -
EL389_RS13550 2514694..2514975 - 282 WP_000603384.1 DNA-binding protein -
EL389_RS13555 2515008..2515769 - 762 WP_000450872.1 DUF1627 domain-containing protein -
EL389_RS13560 2515795..2516536 - 742 Protein_2590 ATP-binding protein -
EL389_RS13565 2516543..2517505 - 963 WP_001444941.1 helix-turn-helix domain-containing protein -
EL389_RS13570 2517528..2517953 - 426 WP_000693943.1 Rha family transcriptional regulator -
EL389_RS13575 2517950..2518252 - 303 WP_000172738.1 transcriptional regulator -
EL389_RS13580 2518350..2518721 + 372 WP_001169687.1 hypothetical protein -
EL389_RS13585 2518742..2518933 + 192 WP_001302048.1 hypothetical protein Antitoxin
EL389_RS13590 2518935..2519213 + 279 WP_001448501.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EL389_RS13595 2519500..2519652 + 153 WP_001427316.1 DUF1391 family protein -
EL389_RS13600 2520073..2520294 + 222 WP_000560218.1 killing protein KilR -
EL389_RS13605 2520288..2520464 + 177 WP_000245534.1 hypothetical protein -
EL389_RS13610 2520538..2520813 + 276 WP_001307773.1 hypothetical protein -
EL389_RS13615 2520912..2523563 + 2652 WP_000105101.1 exodeoxyribonuclease VIII -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 2461117..2539568 78451


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10691.27 Da        Isoelectric Point: 5.5647

>T32374 WP_001448501.1 NZ_AP018808:2518935-2519213 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPSLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T32374 NZ_AP018808:2518935-2519213 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTAGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT32374 WP_001302048.1 NZ_AP018808:2518742-2518933 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT32374 NZ_AP018808:2518742-2518933 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y2Y8G6

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References