Detailed information of TA system TA032373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2157909..2158435
• Replicon: chromosome
• Accession: NZ_AP018808
• Organism: Escherichia coli strain E2865

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: EL389_RS11560
• Protein ID: WP_000323025.1
• Coordinates: 2157909..2158196 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: EL389_RS11565
• Protein ID: WP_000534858.1
• Coordinates: 2158196..2158435 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL389_RS11515	2152933..2153148	-	216	WP_000839590.1	phage lysis protein EssD	-
EL389_RS32265	2153368..2153538	+	171	WP_001405948.1	putative zinc-binding protein YnfU	-
EL389_RS11520	2153902..2154117	-	216	WP_000066483.1	cold shock-like protein CspB	-
EL389_RS11525	2154418..2154630	+	213	WP_000087756.1	cold shock-like protein CspF	-
EL389_RS11530	2154685..2154774	+	90	WP_120795389.1	hypothetical protein	-
EL389_RS11535	2155052..2155804	-	753	WP_001047133.1	antitermination protein	-
EL389_RS11540	2155818..2156867	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
EL389_RS11545	2156869..2157147	-	279	WP_012304870.1	hypothetical protein	-
EL389_RS11550	2157214..2157465	-	252	WP_000980994.1	hypothetical protein	-
EL389_RS11555	2157682..2157837	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
EL389_RS11560	2157909..2158196	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
EL389_RS11565	2158196..2158435	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
EL389_RS11570	2158460..2158765	+	306	WP_001326990.1	hypothetical protein	-
EL389_RS11575	2158968..2159300	+	333	WP_001317460.1	FlxA-like family protein	-
EL389_RS11580	2159368..2160581	+	1214	WP_162830411.1	IS3 family transposase	-
EL389_RS11590	2161050..2162390	-	1341	WP_000589012.1	ISNCY family transposase	-
EL389_RS11595	2162424..2162843	-	420	WP_001151195.1	DUF977 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2114075..2177782	63707

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T32373 WP_000323025.1 NZ_AP018808:c2158196-2157909 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T32373 NZ_AP018808:c2158196-2157909 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT32373 WP_000534858.1 NZ_AP018808:c2158435-2158196 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT32373 NZ_AP018808:c2158435-2158196 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8