32371

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1985263..1985901 Replicon chromosome
Accession NZ_AP018808
Organism Escherichia coli strain E2865

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag EL389_RS10715 Protein ID WP_000813794.1
Coordinates 1985263..1985439 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EL389_RS10720 Protein ID WP_001270286.1
Coordinates 1985485..1985901 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL389_RS10695 1980882..1982096 - 1215 WP_001307190.1 BenE family transporter YdcO -
EL389_RS10700 1982149..1982685 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
EL389_RS10705 1982758..1984719 + 1962 WP_001341357.1 23S rRNA 5-hydroxycytidine C2501 synthase -
EL389_RS10710 1984811..1985041 - 231 WP_000494244.1 YncJ family protein -
EL389_RS10715 1985263..1985439 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
EL389_RS10720 1985485..1985901 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
EL389_RS10725 1985980..1987385 + 1406 Protein_2036 PLP-dependent aminotransferase family protein -
EL389_RS10730 1987630..1988775 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
EL389_RS10735 1988793..1989806 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
EL389_RS10740 1989807..1990748 + 942 WP_001251332.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1979694..1980842 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T32371 WP_000813794.1 NZ_AP018808:1985263-1985439 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T32371 NZ_AP018808:1985263-1985439 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT32371 WP_001270286.1 NZ_AP018808:1985485-1985901 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT32371 NZ_AP018808:1985485-1985901 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References