32359

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/Xre(antitoxin)
Location 32940..33438 Replicon plasmid pE2863-5
Accession NZ_AP018807
Organism Escherichia coli strain E2863

Toxin (Protein)


Gene name TsxA Uniprot ID A0A0K6A0X9
Locus tag EL381_RS29765 Protein ID WP_000702249.1
Coordinates 32940..33206 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID A0A3H7VMJ5
Locus tag EL381_RS29770 Protein ID WP_001450803.1
Coordinates 33196..33438 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL381_RS29720 28057..28344 - 288 WP_000332249.1 helix-turn-helix transcriptional regulator -
EL381_RS29725 28543..28881 + 339 WP_000612441.1 helix-turn-helix domain-containing protein -
EL381_RS29730 29299..30303 - 1005 WP_001208090.1 replication initiation protein -
EL381_RS29750 31981..32304 + 324 WP_000432877.1 hypothetical protein -
EL381_RS29755 32345..32569 + 225 WP_000866040.1 hypothetical protein -
EL381_RS29760 32665..32889 + 225 WP_000749828.1 hypothetical protein -
EL381_RS29765 32940..33206 + 267 WP_000702249.1 hypothetical protein Toxin
EL381_RS29770 33196..33438 + 243 WP_001450803.1 hypothetical protein Antitoxin
EL381_RS29780 33878..34207 + 330 WP_000449468.1 hypothetical protein -
EL381_RS29785 34265..34543 + 279 WP_000545936.1 hypothetical protein -
EL381_RS29790 34986..35168 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin -
EL381_RS29795 35193..35630 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin -
EL381_RS29800 35660..36364 - 705 WP_001279865.1 hypothetical protein -
EL381_RS29805 36361..37122 - 762 WP_001097937.1 conjugal transfer protein TraL -
EL381_RS29810 37132..37539 - 408 WP_001256557.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..37917 37917


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)

Antitoxin

Toxin YoeB

(5-84)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10242.76 Da        Isoelectric Point: 9.9280

>T32359 WP_000702249.1 NZ_AP018807:32940-33206 [Escherichia coli]
MKIRKGDRQYYLNKEGNMFHLVKRVKTFSKSATLGKTKATVITVADLIFHEDAFDTIDFTRGGLRENDKEVVSMMIQEMN
NEREKNVN

Download         Length: 267 bp

>T32359 NZ_AP018807:32940-33206 [Escherichia coli]
ATGAAGATACGAAAAGGTGACAGGCAATATTATTTGAATAAAGAAGGTAATATGTTTCATCTTGTGAAACGGGTGAAGAC
CTTTTCTAAATCTGCGACTTTAGGGAAAACAAAAGCGACAGTGATAACTGTTGCCGATCTAATTTTTCATGAAGATGCAT
TCGACACTATCGACTTTACCAGAGGCGGGTTAAGAGAGAACGACAAAGAAGTTGTTTCTATGATGATTCAGGAAATGAAC
AATGAACGAGAAAAAAATGTTAACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9288.69 Da        Isoelectric Point: 9.7120

>AT32359 WP_001450803.1 NZ_AP018807:33196-33438 [Escherichia coli]
MLTENTPENIKRLRQKIGLTQRECAEIFSMNPRTWRRKEEPAGTASGTALTPVEFKYLQLLAGEHPEYVLRKRDKPKCSE

Download         Length: 243 bp

>AT32359 NZ_AP018807:33196-33438 [Escherichia coli]
ATGTTAACTGAAAATACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACGCAAAGAGAATGTGCTGAAAT
ATTCAGCATGAACCCGCGAACCTGGCGTCGCAAGGAAGAACCTGCCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATATCTTCAGCTTTTAGCTGGGGAACATCCTGAATACGTCCTGCGCAAAAGGGATAAACCTAAATGTAGTGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K6A0X9

Antitoxin

Source ID Structure
AlphaFold DB A0A3H7VMJ5

References