Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5024620..5025117 Replicon chromosome
Accession NZ_AP018802
Organism Escherichia coli strain E2863

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EL381_RS25525 Protein ID WP_001173343.1
Coordinates 5024944..5025117 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EL381_RS25520 Protein ID WP_000398619.1
Coordinates 5024620..5024916 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL381_RS25500 5021351..5022070 - 720 WP_000611299.1 two-component system response regulator DcuR -
EL381_RS25505 5022067..5023698 - 1632 WP_001216487.1 sensor histidine kinase -
EL381_RS25510 5023879..5024109 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
EL381_RS25515 5024121..5024393 + 273 WP_000405647.1 N-acetyltransferase -
EL381_RS25520 5024620..5024916 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EL381_RS25525 5024944..5025117 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EL381_RS25530 5025236..5025976 - 741 Protein_4894 lysine--tRNA ligase -
EL381_RS25535 5026010..5027223 + 1214 WP_162829202.1 IS3 family transposase -
EL381_RS25540 5027267..5027425 - 159 Protein_4896 IS256 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T32347 WP_001173343.1 NZ_AP018802:5024944-5025117 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T32347 NZ_AP018802:5024944-5025117 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT32347 WP_000398619.1 NZ_AP018802:5024620-5024916 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT32347 NZ_AP018802:5024620-5024916 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References