Detailed information of TA system TA032341

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3103677..3104302
• Replicon: chromosome
• Accession: NZ_AP018802
• Organism: Escherichia coli strain E2863

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EL381_RS16075
• Protein ID: WP_000911330.1
• Coordinates: 3103677..3104075 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EL381_RS16080
• Protein ID: WP_000450524.1
• Coordinates: 3104075..3104302 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL381_RS16055	3099555..3099755	+	201	WP_000383836.1	YpfN family protein	-
EL381_RS16060	3099865..3100563	-	699	WP_000679812.1	esterase	-
EL381_RS16065	3100637..3102652	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
EL381_RS16070	3102667..3103530	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
EL381_RS16075	3103677..3104075	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EL381_RS16080	3104075..3104302	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EL381_RS16085	3104456..3105169	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EL381_RS16090	3105382..3106416	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
EL381_RS16095	3106433..3107311	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EL381_RS16100	3107457..3108041	+	585	WP_000176194.1	glycine cleavage system transcriptional repressor	-
EL381_RS16105	3108041..3108511	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T32341 WP_000911330.1 NZ_AP018802:c3104075-3103677 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T32341 NZ_AP018802:c3104075-3103677 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT32341 WP_000450524.1 NZ_AP018802:c3104302-3104075 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT32341 NZ_AP018802:c3104302-3104075 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33