Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1873770..1874408 Replicon chromosome
Accession NZ_AP018802
Organism Escherichia coli strain E2863

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag EL381_RS09555 Protein ID WP_000813794.1
Coordinates 1874232..1874408 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EL381_RS09550 Protein ID WP_001270286.1
Coordinates 1873770..1874186 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL381_RS09530 1868922..1869863 - 942 WP_001251315.1 ABC transporter permease -
EL381_RS09535 1869864..1870877 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
EL381_RS09540 1870895..1872040 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
EL381_RS09545 1872285..1873691 - 1407 WP_000760637.1 PLP-dependent aminotransferase family protein -
EL381_RS09550 1873770..1874186 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
EL381_RS09555 1874232..1874408 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
EL381_RS09560 1874630..1874860 + 231 WP_000494244.1 YncJ family protein -
EL381_RS09565 1874952..1876913 - 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
EL381_RS09570 1876986..1877522 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
EL381_RS09575 1877575..1878783 + 1209 WP_071532666.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1878823..1879971 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T32331 WP_000813794.1 NZ_AP018802:c1874408-1874232 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T32331 NZ_AP018802:c1874408-1874232 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT32331 WP_001270286.1 NZ_AP018802:c1874186-1873770 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT32331 NZ_AP018802:c1874186-1873770 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References