Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1352308..1352779 Replicon chromosome
Accession NZ_AP018802
Organism Escherichia coli strain E2863

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag EL381_RS06685 Protein ID WP_001303511.1
Coordinates 1352308..1352586 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag EL381_RS06690 Protein ID WP_001302048.1
Coordinates 1352588..1352779 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL381_RS06650 1347615..1347884 - 270 WP_000003742.1 excisionase -
EL381_RS06655 1347946..1350411 - 2466 WP_000048442.1 exonuclease -
EL381_RS06660 1350504..1350695 - 192 WP_001090200.1 DUF1482 family protein -
EL381_RS06665 1350692..1350880 - 189 WP_000449192.1 cell division inhibition protein DicB -
EL381_RS06675 1351364..1351582 - 219 WP_001171930.1 hypothetical protein -
EL381_RS06680 1351623..1352012 - 390 WP_001447517.1 hypothetical protein -
EL381_RS06685 1352308..1352586 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EL381_RS06690 1352588..1352779 - 192 WP_001302048.1 hypothetical protein Antitoxin
EL381_RS06695 1352800..1353171 - 372 WP_001169687.1 hypothetical protein -
EL381_RS06705 1354575..1354715 + 141 WP_072058819.1 type I toxin-antitoxin system Hok family toxin -
EL381_RS06715 1354883..1355155 + 273 WP_012817871.1 hypothetical protein -
EL381_RS06720 1355157..1356212 + 1056 WP_001369253.1 DUF968 domain-containing protein -
EL381_RS06725 1356213..1356578 + 366 WP_000140024.1 RusA family crossover junction endodeoxyribonuclease -
EL381_RS06730 1356587..1357117 + 531 WP_000640048.1 DUF1133 family protein -
EL381_RS06735 1357359..1357556 + 198 WP_000917750.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1346528..1389210 42682


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T32325 WP_001303511.1 NZ_AP018802:c1352586-1352308 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T32325 NZ_AP018802:c1352586-1352308 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT32325 WP_001302048.1 NZ_AP018802:c1352779-1352588 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT32325 NZ_AP018802:c1352779-1352588 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References