Detailed information of TA system TA032310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 86009..86610
• Replicon: plasmid pE2855-2
• Accession: NZ_AP018798
• Organism: Escherichia coli strain E2855

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: MCREHEC_RS29450
• Protein ID: WP_001216045.1
• Coordinates: 86230..86610 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: MCREHEC_RS29445
• Protein ID: WP_001190712.1
• Coordinates: 86009..86230 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCREHEC_RS29390	81104..81361	+	258	WP_000382636.1	hypothetical protein	-
MCREHEC_RS29395	81361..81642	+	282	WP_000002126.1	ASCH domain-containing protein	-
MCREHEC_RS29400	81666..81959	+	294	WP_000267998.1	hypothetical protein	-
MCREHEC_RS29405	81966..82340	+	375	WP_000988652.1	hypothetical protein	-
MCREHEC_RS29410	82322..82996	+	675	WP_000057460.1	hypothetical protein	-
MCREHEC_RS29415	82993..83355	+	363	WP_001261541.1	hypothetical protein	-
MCREHEC_RS29430	84431..84787	-	357	WP_001062547.1	hypothetical protein	-
MCREHEC_RS29435	84788..85372	-	585	WP_001133673.1	hypothetical protein	-
MCREHEC_RS29440	85547..85936	+	390	WP_000506724.1	DNA repair protein	-
MCREHEC_RS29445	86009..86230	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MCREHEC_RS29450	86230..86610	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MCREHEC_RS29455	86615..86794	+	180	WP_000113018.1	hypothetical protein	-
MCREHEC_RS29460	86822..87865	+	1044	WP_000648833.1	DUF968 domain-containing protein	-
MCREHEC_RS29465	87954..88406	+	453	WP_001326849.1	late promoter-activating protein	-
MCREHEC_RS29470	88492..89685	+	1194	WP_097463274.1	terminase	-
MCREHEC_RS29475	89685..91169	+	1485	WP_000124153.1	hypothetical protein	-
MCREHEC_RS31780	91274..91504	+	231	Protein_103	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94446	94446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T32310 WP_001216045.1 NZ_AP018798:86230-86610 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T32310 NZ_AP018798:86230-86610 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT32310 WP_001190712.1 NZ_AP018798:86009-86230 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT32310 NZ_AP018798:86009-86230 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6