Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 5301483..5302078 Replicon chromosome
Accession NZ_AP018796
Organism Escherichia coli strain E2855

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag MCREHEC_RS27260 Protein ID WP_000239579.1
Coordinates 5301728..5302078 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag MCREHEC_RS27255 Protein ID WP_001223208.1
Coordinates 5301483..5301734 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCREHEC_RS27245 5297148..5300927 + 3780 WP_000060925.1 autotransporter assembly complex protein TamB -
MCREHEC_RS27250 5300930..5301271 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
MCREHEC_RS27255 5301483..5301734 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
MCREHEC_RS27260 5301728..5302078 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
MCREHEC_RS27265 5302158..5302688 - 531 WP_000055075.1 inorganic diphosphatase -
MCREHEC_RS27270 5302998..5303954 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
MCREHEC_RS27275 5304094..5305596 + 1503 WP_000205815.1 sugar ABC transporter ATP-binding protein -
MCREHEC_RS27280 5305610..5306632 + 1023 WP_001296689.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T32308 WP_000239579.1 NZ_AP018796:5301728-5302078 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T32308 NZ_AP018796:5301728-5302078 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT32308 WP_001223208.1 NZ_AP018796:5301483-5301734 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT32308 NZ_AP018796:5301483-5301734 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References