32304

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3686629..3687283 Replicon chromosome
Accession NZ_AP018796
Organism Escherichia coli strain E2855

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag MCREHEC_RS19265 Protein ID WP_000244777.1
Coordinates 3686629..3687036 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MCREHEC_RS19270 Protein ID WP_000354046.1
Coordinates 3687017..3687283 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCREHEC_RS19245 3682586..3684319 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
MCREHEC_RS19250 3684325..3685035 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MCREHEC_RS19255 3685060..3685956 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MCREHEC_RS19260 3686068..3686589 + 522 WP_001055874.1 flavodoxin FldB -
MCREHEC_RS19265 3686629..3687036 - 408 WP_000244777.1 toxin CptA Toxin
MCREHEC_RS19270 3687017..3687283 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MCREHEC_RS19275 3687526..3688506 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
MCREHEC_RS19280 3688702..3689361 - 660 WP_000250274.1 hemolysin III family protein -
MCREHEC_RS19285 3689525..3689836 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
MCREHEC_RS19290 3689881..3691314 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
MCREHEC_RS19295 3691371..3692114 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T32304 WP_000244777.1 NZ_AP018796:c3687036-3686629 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T32304 NZ_AP018796:c3687036-3686629 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT32304 WP_000354046.1 NZ_AP018796:c3687283-3687017 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT32304 NZ_AP018796:c3687283-3687017 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QD57

References