Detailed information of TA system TA032301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3176017..3176642
• Replicon: chromosome
• Accession: NZ_AP018796
• Organism: Escherichia coli strain E2855

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MCREHEC_RS16575
• Protein ID: WP_000911330.1
• Coordinates: 3176017..3176415 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: MCREHEC_RS16580
• Protein ID: WP_000450524.1
• Coordinates: 3176415..3176642 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCREHEC_RS16555	3171895..3172095	+	201	WP_000383836.1	YpfN family protein	-
MCREHEC_RS16560	3172205..3172903	-	699	WP_000679812.1	esterase	-
MCREHEC_RS16565	3172977..3174992	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
MCREHEC_RS16570	3175007..3175870	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
MCREHEC_RS16575	3176017..3176415	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
MCREHEC_RS16580	3176415..3176642	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
MCREHEC_RS16585	3176796..3177509	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
MCREHEC_RS16590	3177722..3178756	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
MCREHEC_RS16595	3178773..3179651	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
MCREHEC_RS16600	3179797..3180387	+	591	WP_106914110.1	glycine cleavage system transcriptional repressor	-
MCREHEC_RS16605	3180387..3180857	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T32301 WP_000911330.1 NZ_AP018796:c3176415-3176017 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T32301 NZ_AP018796:c3176415-3176017 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT32301 WP_000450524.1 NZ_AP018796:c3176642-3176415 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT32301 NZ_AP018796:c3176642-3176415 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33