Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1839798..1840436 Replicon chromosome
Accession NZ_AP018796
Organism Escherichia coli strain E2855

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag MCREHEC_RS09500 Protein ID WP_000813794.1
Coordinates 1839798..1839974 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag MCREHEC_RS09505 Protein ID WP_001270286.1
Coordinates 1840020..1840436 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCREHEC_RS09480 1835423..1836631 - 1209 WP_071532666.1 BenE family transporter YdcO -
MCREHEC_RS09485 1836684..1837220 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
MCREHEC_RS09490 1837293..1839254 + 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
MCREHEC_RS09495 1839346..1839576 - 231 WP_000494244.1 YncJ family protein -
MCREHEC_RS09500 1839798..1839974 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
MCREHEC_RS09505 1840020..1840436 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
MCREHEC_RS09510 1840515..1841921 + 1407 WP_000760637.1 PLP-dependent aminotransferase family protein -
MCREHEC_RS09515 1842166..1843311 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
MCREHEC_RS09520 1843329..1844342 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
MCREHEC_RS09525 1844343..1845284 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1834235..1835383 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T32290 WP_000813794.1 NZ_AP018796:1839798-1839974 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T32290 NZ_AP018796:1839798-1839974 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT32290 WP_001270286.1 NZ_AP018796:1840020-1840436 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT32290 NZ_AP018796:1840020-1840436 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References