Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1423954..1424425 Replicon chromosome
Accession NZ_AP018796
Organism Escherichia coli strain E2855

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag MCREHEC_RS07225 Protein ID WP_001303511.1
Coordinates 1423954..1424232 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag MCREHEC_RS07230 Protein ID WP_001302048.1
Coordinates 1424234..1424425 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCREHEC_RS07190 1419261..1419530 - 270 WP_000003742.1 excisionase -
MCREHEC_RS07195 1419592..1422057 - 2466 WP_000048442.1 exonuclease -
MCREHEC_RS07200 1422150..1422341 - 192 WP_001090200.1 DUF1482 family protein -
MCREHEC_RS07205 1422338..1422526 - 189 WP_000449192.1 cell division inhibition protein DicB -
MCREHEC_RS07215 1423010..1423228 - 219 WP_001171930.1 hypothetical protein -
MCREHEC_RS07220 1423269..1423658 - 390 WP_001447517.1 hypothetical protein -
MCREHEC_RS07225 1423954..1424232 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MCREHEC_RS07230 1424234..1424425 - 192 WP_001302048.1 hypothetical protein Antitoxin
MCREHEC_RS07235 1424446..1424817 - 372 WP_001169687.1 hypothetical protein -
MCREHEC_RS07240 1424916..1425218 + 303 WP_001444689.1 transcriptional regulator -
MCREHEC_RS07245 1425215..1425640 + 426 WP_000693943.1 Rha family transcriptional regulator -
MCREHEC_RS07250 1425663..1426625 + 963 WP_000095671.1 helix-turn-helix domain-containing protein -
MCREHEC_RS07255 1426666..1427082 + 417 WP_023981635.1 DUF977 family protein -
MCREHEC_RS07265 1428373..1428513 + 141 WP_072058819.1 type I toxin-antitoxin system Hok family toxin -
MCREHEC_RS07275 1428681..1428953 + 273 WP_012817871.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espX7/nleL / espN / espK 1418174..1472265 54091


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T32285 WP_001303511.1 NZ_AP018796:c1424232-1423954 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T32285 NZ_AP018796:c1424232-1423954 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATTCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT32285 WP_001302048.1 NZ_AP018796:c1424425-1424234 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT32285 NZ_AP018796:c1424425-1424234 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References