Detailed information of TA system TA032280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 2612996..2613591
• Replicon: chromosome
• Accession: NZ_AP018795
• Organism: Acidithiobacillus ferridurans strain JCM 18981

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: AFERRID_RS13485
• Protein ID: WP_126605735.1
• Coordinates: 2612996..2613292 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: AFERRID_RS13490
• Protein ID: WP_126605470.1
• Coordinates: 2613295..2613591 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFERRID_RS13440	2608335..2608742	-	408	WP_126605462.1	hypothetical protein	-
AFERRID_RS15335	2608961..2609089	+	129	WP_197722445.1	BrnT family toxin	-
AFERRID_RS13450	2609193..2609513	+	321	WP_126605463.1	BrnA antitoxin family protein	-
AFERRID_RS13455	2609522..2610112	-	591	WP_126605464.1	S26 family signal peptidase	-
AFERRID_RS13460	2610114..2610605	-	492	WP_126605465.1	hypothetical protein	-
AFERRID_RS13465	2610630..2610845	-	216	WP_126605466.1	hypothetical protein	-
AFERRID_RS13470	2611012..2611275	-	264	WP_126605467.1	hypothetical protein	-
AFERRID_RS13480	2611595..2612821	-	1227	WP_126605469.1	conjugal transfer protein TraB	-
AFERRID_RS13485	2612996..2613292	+	297	WP_126605735.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AFERRID_RS13490	2613295..2613591	+	297	WP_126605470.1	putative addiction module antidote protein	Antitoxin
AFERRID_RS13495	2614155..2614838	-	684	WP_126605471.1	hypothetical protein	-
AFERRID_RS13500	2615184..2615474	-	291	WP_126605472.1	hypothetical protein	-
AFERRID_RS13505	2615471..2617519	-	2049	WP_126605473.1	DNA topoisomerase 3	-
AFERRID_RS13510	2617686..2618117	+	432	WP_126605474.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2590114..2631127	41013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (11-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11141.94 Da
• Isoelectric Point: 10.0301

>T32280 WP_126605735.1 NZ_AP018795:2612996-2613292 [Acidithiobacillus ferridurans]
MVEIKQTETFRKWWMKLKDERARGIVAGRLDRLAYGLIGDAEPVGEGVSELRIHYGPGYRVYFQQRGSMIIVLLCGGNKS
TQAKDIKVAKRLAAEWSE

Nucleotide

• Length: 297 bp

>T32280 NZ_AP018795:2612996-2613292 [Acidithiobacillus ferridurans]
ATGGTTGAGATCAAGCAGACAGAGACCTTCAGAAAATGGTGGATGAAGCTCAAAGATGAACGAGCTCGCGGTATCGTGGC
CGGTCGTCTGGATCGGCTCGCTTACGGCTTGATCGGCGACGCGGAGCCAGTGGGGGAGGGGGTAAGTGAGTTACGCATCC
ACTATGGCCCTGGATACCGTGTTTATTTTCAGCAACGGGGGAGCATGATCATCGTCTTGCTCTGCGGTGGCAACAAGAGC
ACGCAAGCGAAGGACATCAAGGTGGCGAAGCGCCTGGCTGCTGAATGGAGTGAATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10225.61 Da
• Isoelectric Point: 5.8085

>AT32280 WP_126605470.1 NZ_AP018795:2613295-2613591 [Acidithiobacillus ferridurans]
MSEKLSTFDPAEGLTSDAAIAAFMADAFESADAGYIAHALGVVARAKGMAQIAGETGLSREQLYRSFSERGNPTLKTTLA
VMKVLGIELTAKTGSQGR

Nucleotide

• Length: 297 bp

>AT32280 NZ_AP018795:2613295-2613591 [Acidithiobacillus ferridurans]
ATGAGTGAAAAATTGTCTACTTTTGACCCTGCCGAAGGGCTGACATCAGACGCGGCCATCGCAGCCTTCATGGCCGATGC
GTTTGAATCTGCAGACGCCGGCTACATTGCGCACGCGCTGGGCGTCGTGGCCCGCGCCAAGGGAATGGCTCAGATCGCTG
GGGAAACTGGTCTTTCTCGTGAACAGTTGTACCGCTCATTCAGCGAGCGCGGCAATCCGACGCTCAAGACCACGCTGGCA
GTCATGAAGGTCCTGGGGATTGAACTTACGGCAAAAACGGGTTCACAGGGCCGATAA