Detailed information of TA system TA032279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapB(antitoxin)
• Location: 2316308..2316876
• Replicon: chromosome
• Accession: NZ_AP018795
• Organism: Acidithiobacillus ferridurans strain JCM 18981

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AFERRID_RS11945
• Protein ID: WP_126605280.1
• Coordinates: 2316308..2316682 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2Z6IL32
• Locus tag: AFERRID_RS11950
• Protein ID: WP_012536620.1
• Coordinates: 2316679..2316876 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFERRID_RS11935	2313552..2314592	-	1041	WP_197722437.1	restriction endonuclease subunit S	-
AFERRID_RS11940	2314754..2316208	-	1455	WP_126605279.1	type I restriction-modification system subunit M	-
AFERRID_RS11945	2316308..2316682	-	375	WP_126605280.1	type II toxin-antitoxin system VapC family toxin	Toxin
AFERRID_RS11950	2316679..2316876	-	198	WP_012536620.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AFERRID_RS15310	2317232..2317411	-	180	WP_197722438.1	hypothetical protein	-
AFERRID_RS11955	2317745..2318419	+	675	WP_126605281.1	hypothetical protein	-
AFERRID_RS11960	2318416..2318739	+	324	WP_126605282.1	integration host factor subunit alpha	-
AFERRID_RS11965	2318806..2319081	+	276	WP_126605283.1	HU family DNA-binding protein	-
AFERRID_RS11970	2319093..2319440	+	348	WP_126605284.1	hypothetical protein	-
AFERRID_RS11975	2319449..2319670	+	222	WP_009560828.1	HU family DNA-binding protein	-
AFERRID_RS11985	2320273..2320407	-	135	Protein_2321	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
AFERRID_RS11990	2320394..2320663	-	270	WP_126605285.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
AFERRID_RS11995	2320688..2321584	-	897	WP_126605286.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2295375..2464924	169549

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-109)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13671.76 Da
• Isoelectric Point: 6.3136

>T32279 WP_126605280.1 NZ_AP018795:c2316682-2316308 [Acidithiobacillus ferridurans]
MILVDTSVWIDHFRSGDAHLSTLLDHAMVQTHDFIIGEIACGNLKNRQQTLYLLSCLPRCTATSPEETLFFIGRHGLMGR
GIGYIDASILASAMLVQANLWSRDKRLMALATELGCAYSPTSSE

Nucleotide

• Length: 375 bp

>T32279 NZ_AP018795:c2316682-2316308 [Acidithiobacillus ferridurans]
TTGATTCTGGTCGATACCTCCGTCTGGATCGACCATTTCCGCTCTGGAGATGCACACCTCTCTACTCTGCTTGATCATGC
CATGGTGCAAACGCACGATTTCATCATCGGAGAAATCGCTTGCGGGAATCTCAAAAATCGGCAACAGACCTTATATCTGC
TCTCATGTTTACCCCGATGCACCGCCACCTCTCCAGAAGAAACCCTGTTTTTCATTGGGCGTCATGGACTGATGGGACGC
GGTATAGGCTATATTGACGCGAGTATCCTAGCCTCCGCCATGCTCGTGCAGGCCAATTTATGGTCCCGCGACAAACGTCT
CATGGCTCTGGCTACCGAACTTGGCTGCGCGTACTCGCCGACGTCATCCGAATAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7266.37 Da
• Isoelectric Point: 9.4896

>AT32279 WP_012536620.1 NZ_AP018795:c2316876-2316679 [Acidithiobacillus ferridurans]
MRTTINLDDELLAQAKTLCGVEDRGALLKEALRALIERESARRLARLGGTEPQLQNIPRRREPAA

Nucleotide

• Length: 198 bp

>AT32279 NZ_AP018795:c2316876-2316679 [Acidithiobacillus ferridurans]
ATGCGAACGACAATCAACCTGGATGATGAGCTCCTGGCCCAAGCCAAAACGCTCTGTGGCGTAGAAGACCGTGGTGCCCT
CTTAAAAGAGGCCCTGCGTGCTTTGATTGAACGGGAAAGTGCCCGTCGCCTCGCTCGCTTGGGTGGAACAGAACCGCAGT
TACAGAACATTCCCCGCAGACGAGAGCCTGCCGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2Z6IL32