Detailed information of TA system TA032276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1301078..1301751
• Replicon: chromosome
• Accession: NZ_AP018795
• Organism: Acidithiobacillus ferridurans strain JCM 18981

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AFERRID_RS06750
• Protein ID: WP_126604657.1
• Coordinates: 1301329..1301751 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AFERRID_RS06745
• Protein ID: WP_113526150.1
• Coordinates: 1301078..1301332 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFERRID_RS06705	1296241..1296498	-	258	WP_126604650.1	hypothetical protein	-
AFERRID_RS06710	1296495..1296794	-	300	WP_126605683.1	nucleotidyltransferase domain-containing protein	-
AFERRID_RS06715	1296800..1297360	-	561	WP_126604651.1	Uma2 family endonuclease	-
AFERRID_RS06720	1297357..1297716	-	360	WP_126604652.1	MarR family EPS-associated transcriptional regulator	-
AFERRID_RS06725	1297804..1298628	-	825	WP_126604653.1	chain-length determining protein	-
AFERRID_RS06730	1298848..1300266	+	1419	WP_172959406.1	sugar transferase	-
AFERRID_RS06735	1300337..1300591	-	255	WP_126604655.1	Txe/YoeB family addiction module toxin	-
AFERRID_RS06740	1300588..1300839	-	252	WP_126604656.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
AFERRID_RS06745	1301078..1301332	+	255	WP_113526150.1	plasmid stabilization protein	Antitoxin
AFERRID_RS06750	1301329..1301751	+	423	WP_126604657.1	type II toxin-antitoxin system VapC family toxin	Toxin
AFERRID_RS06755	1301935..1302512	+	578	Protein_1303	peptidoglycan-associated lipoprotein Pal	-
AFERRID_RS06760	1302533..1303351	+	819	WP_113526373.1	tol-pal system protein YbgF	-
AFERRID_RS06765	1303351..1303995	+	645	WP_126604658.1	7-carboxy-7-deazaguanine synthase QueE	-
AFERRID_RS06770	1303998..1304687	+	690	WP_113526146.1	7-cyano-7-deazaguanine synthase QueC	-
AFERRID_RS06775	1304729..1305996	-	1268	Protein_1307	Tol-Pal system beta propeller repeat protein TolB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14981.10 Da
• Isoelectric Point: 4.6672

>T32276 WP_126604657.1 NZ_AP018795:1301329-1301751 [Acidithiobacillus ferridurans]
MIVLDTNVVSEAMKAEPNSAVRVWLDDQAAETLYLSSITLAELLFGIGALPSGRRKDALTQTLDGLLELFGDRVLAFDTD
AARHYVELAVNARAASKGFPTPDGHIAAIANARGFTVATRDVAPFLAAGLNVINPWEAMR

Nucleotide

• Length: 423 bp

>T32276 NZ_AP018795:1301329-1301751 [Acidithiobacillus ferridurans]
ATGATCGTCTTGGATACCAACGTCGTTTCCGAGGCGATGAAAGCGGAACCGAATTCCGCCGTGCGGGTATGGCTGGATGA
TCAGGCGGCCGAAACCCTCTACCTGTCCAGCATCACGCTTGCCGAACTGCTGTTCGGCATCGGTGCCCTGCCTTCCGGCC
GACGCAAAGATGCGCTGACCCAAACGCTGGATGGCCTATTGGAGCTGTTCGGGGATCGCGTGCTGGCCTTCGACACCGAC
GCAGCCCGGCACTACGTCGAGCTGGCCGTTAACGCCCGTGCCGCCAGCAAGGGTTTCCCCACACCAGACGGCCACATCGC
CGCCATTGCGAACGCGCGCGGCTTCACGGTGGCCACGCGCGACGTTGCGCCGTTCCTGGCGGCCGGCCTCAACGTCATCA
ACCCGTGGGAGGCCATGCGGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9417.68 Da
• Isoelectric Point: 9.0109

>AT32276 WP_113526150.1 NZ_AP018795:1301078-1301332 [Acidithiobacillus ferridurans]
MATVTVRNLPDEVHRALRVRAATHGRSTEAEIRDILESTVRPPKRLRLGTALAELGRRAGLTDDDIATFEQVRDKTPAEP
VRFE

Nucleotide

• Length: 255 bp

>AT32276 NZ_AP018795:1301078-1301332 [Acidithiobacillus ferridurans]
ATGGCGACAGTTACCGTCAGAAATCTGCCGGACGAGGTGCATCGCGCGCTGCGTGTACGCGCAGCAACGCACGGCCGCAG
CACCGAGGCCGAAATCCGCGACATCCTCGAATCGACCGTTCGCCCGCCAAAGCGGCTTCGCCTGGGCACGGCCCTGGCCG
AGCTGGGCCGCCGCGCCGGGCTGACCGATGACGACATCGCAACCTTCGAGCAGGTGCGCGACAAGACCCCGGCCGAGCCC
GTGAGGTTTGAATGA