Detailed information of TA system TA032274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapB(antitoxin)
• Location: 1230911..1231479
• Replicon: chromosome
• Accession: NZ_AP018795
• Organism: Acidithiobacillus ferridurans strain JCM 18981

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AFERRID_RS06340
• Protein ID: WP_126604600.1
• Coordinates: 1231105..1231479 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2Z6IL32
• Locus tag: AFERRID_RS06335
• Protein ID: WP_012536620.1
• Coordinates: 1230911..1231108 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFERRID_RS06295	1225949..1226332	+	384	WP_113526224.1	DUF423 domain-containing protein	-
AFERRID_RS06300	1226363..1226761	+	399	WP_012535979.1	rhodanese-like domain-containing protein	-
AFERRID_RS06305	1226875..1227669	+	795	WP_126604595.1	transferase	-
AFERRID_RS06310	1227878..1228123	+	246	WP_113526222.1	hypothetical protein	-
AFERRID_RS06315	1228525..1228785	+	261	WP_126604596.1	deoxyribodipyrimidine photo-lyase	-
AFERRID_RS06320	1229056..1230129	+	1074	WP_126604597.1	alkene reductase	-
AFERRID_RS06325	1230350..1230619	+	270	WP_126604598.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
AFERRID_RS06330	1230606..1230974	+	369	WP_126604599.1	type II toxin-antitoxin system YafQ family toxin	-
AFERRID_RS06335	1230911..1231108	+	198	WP_012536620.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AFERRID_RS06340	1231105..1231479	+	375	WP_126604600.1	type II toxin-antitoxin system VapC family toxin	Toxin
AFERRID_RS06345	1231578..1233032	+	1455	WP_126604601.1	type I restriction-modification system subunit M	-
AFERRID_RS06350	1233029..1233466	+	438	WP_126604602.1	restriction endonuclease subunit S	-
AFERRID_RS06355	1233577..1234170	+	594	WP_172959342.1	restriction endonuclease subunit S	-
AFERRID_RS06360	1234181..1236477	+	2297	Protein_1227	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-109)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13701.78 Da
• Isoelectric Point: 6.3136

>T32274 WP_126604600.1 NZ_AP018795:1231105-1231479 [Acidithiobacillus ferridurans]
MILVDTSVWIDHFRSGDAHLSTLLDHAMVQTHDFIIGEIACGNLKNRQQTLYLLSCLPRCTATSPEETLFFIGRHSLMGR
GIGYIDASILASAMLVQANLWSRDKRLMALATELGCAYSPTSSE

Nucleotide

• Length: 375 bp

>T32274 NZ_AP018795:1231105-1231479 [Acidithiobacillus ferridurans]
TTGATTCTGGTCGATACCTCCGTCTGGATCGACCATTTCCGCTCTGGAGATGCACACCTCTCTACTCTGCTTGATCATGC
CATGGTGCAAACGCACGATTTCATCATCGGAGAAATCGCTTGCGGGAATCTCAAAAATCGGCAACAGACCTTATATCTGC
TCTCCTGTTTACCCCGATGCACCGCCACCTCTCCAGAAGAAACCCTGTTTTTCATTGGGCGTCATAGCCTGATGGGACGC
GGTATAGGCTATATTGACGCGAGTATCCTAGCCTCCGCCATGCTCGTGCAGGCCAATTTATGGTCCCGCGACAAACGTCT
CATGGCTCTGGCTACCGAACTTGGCTGCGCGTACTCGCCGACGTCATCCGAATAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7266.37 Da
• Isoelectric Point: 9.4896

>AT32274 WP_012536620.1 NZ_AP018795:1230911-1231108 [Acidithiobacillus ferridurans]
MRTTINLDDELLAQAKTLCGVEDRGALLKEALRALIERESARRLARLGGTEPQLQNIPRRREPAA

Nucleotide

• Length: 198 bp

>AT32274 NZ_AP018795:1230911-1231108 [Acidithiobacillus ferridurans]
ATGCGAACGACAATCAACCTGGATGATGAGCTCCTGGCCCAAGCCAAAACGCTCTGTGGCGTAGAAGACCGTGGTGCCCT
CTTAAAAGAGGCCCTGCGTGCTTTGATTGAACGGGAAAGTGCCCGTCGCCTCGCTCGCTTGGGTGGAACAGAACCGCAAT
TACAGAACATTCCTCGCAGACGAGAGCCTGCCGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2Z6IL32