Detailed information of TA system TA032273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 911587..912167
• Replicon: chromosome
• Accession: NZ_AP018795
• Organism: Acidithiobacillus ferridurans strain JCM 18981

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: AFERRID_RS04600
• Protein ID: WP_126604462.1
• Coordinates: 911841..912167 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: AFERRID_RS04595
• Protein ID: WP_126604461.1
• Coordinates: 911587..911844 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFERRID_RS04580	907971..909485	+	1515	WP_126604458.1	hypothetical protein	-
AFERRID_RS04585	909596..910618	+	1023	WP_126604459.1	GDP-mannose 4,6-dehydratase	-
AFERRID_RS04590	910635..911474	+	840	WP_126604460.1	methyltransferase domain-containing protein	-
AFERRID_RS04595	911587..911844	+	258	WP_126604461.1	antitoxin	Antitoxin
AFERRID_RS04600	911841..912167	+	327	WP_126604462.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AFERRID_RS15145	912175..912315	+	141	WP_172959336.1	hypothetical protein	-
AFERRID_RS04605	912396..912626	-	231	WP_126604463.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
AFERRID_RS04610	912630..912767	-	138	WP_197722480.1	hypothetical protein	-
AFERRID_RS04615	912802..913182	-	381	WP_126604464.1	SCP2 sterol-binding domain-containing protein	-
AFERRID_RS04620	913319..913615	+	297	WP_126605675.1	DUF1778 domain-containing protein	-
AFERRID_RS04625	913616..914134	+	519	WP_126604465.1	GNAT family N-acetyltransferase	-
AFERRID_RS04630	914286..914882	+	597	WP_192796491.1	ATP-binding cassette domain-containing protein	-
AFERRID_RS04635	914882..915643	+	762	WP_126604467.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	902248..941655	39407

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-107)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11713.64 Da
• Isoelectric Point: 8.0184

>T32273 WP_126604462.1 NZ_AP018795:911841-912167 [Acidithiobacillus ferridurans]
MKRGDIYLVSLDPTAGHEQSGSRPVLIVSPAEFNETTKLPVILPITNGGEFARRLGFAVPVTGIKTTGVVRCDQPRVMDL
AARNARKVDTLPVPIMDEVLARVATIFE

Nucleotide

• Length: 327 bp

>T32273 NZ_AP018795:911841-912167 [Acidithiobacillus ferridurans]
ATGAAGCGAGGTGACATCTACCTCGTGTCGCTCGATCCCACCGCCGGGCATGAACAAAGCGGTAGCCGTCCTGTCCTGAT
CGTGTCGCCTGCGGAGTTTAATGAAACCACCAAGCTGCCGGTGATCCTACCGATCACCAATGGTGGTGAGTTCGCCCGTC
GCCTTGGTTTCGCGGTGCCCGTGACCGGCATAAAGACCACCGGCGTCGTGCGCTGCGACCAGCCGCGCGTGATGGACTTG
GCCGCCCGAAATGCCCGCAAGGTGGACACTCTGCCGGTACCGATCATGGACGAGGTGCTGGCCAGGGTCGCCACGATCTT
CGAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9123.55 Da
• Isoelectric Point: 5.5255

>AT32273 WP_126604461.1 NZ_AP018795:911587-911844 [Acidithiobacillus ferridurans]
MHTTKLRKVGGSIMLAVPPAFLEQLHLQAGATVGLVVDHGLLVVNPSPRPRYTLAELLAASDYSQPQPVEEREWVDAPAV
GGELL

Nucleotide

• Length: 258 bp

>AT32273 NZ_AP018795:911587-911844 [Acidithiobacillus ferridurans]
ATGCACACAACCAAACTGCGCAAGGTCGGCGGCTCGATCATGTTGGCCGTCCCACCCGCATTCCTTGAACAGCTGCACCT
GCAAGCCGGTGCGACGGTCGGCTTGGTCGTCGATCATGGTCTACTGGTGGTCAATCCCAGTCCACGCCCGCGCTACACAC
TTGCAGAGTTGCTGGCCGCGTCCGACTATTCCCAGCCGCAACCGGTCGAGGAGCGCGAATGGGTCGATGCGCCCGCCGTG
GGCGGTGAGCTGCTATGA