Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 129353..129878 Replicon plasmid pSK1144
Accession NZ_AP018785
Organism Escherichia coli strain SK1144

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag DAECLI1_RS25765 Protein ID WP_001159871.1
Coordinates 129573..129878 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag DAECLI1_RS25760 Protein ID WP_000813634.1
Coordinates 129353..129571 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DAECLI1_RS25750 126390..127031 - 642 WP_000990667.1 hypothetical protein -
DAECLI1_RS25760 129353..129571 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DAECLI1_RS25765 129573..129878 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
DAECLI1_RS25770 129879..130688 + 810 WP_000016979.1 site-specific integrase -
DAECLI1_RS25775 130826..131101 - 276 WP_000239529.1 hypothetical protein -
DAECLI1_RS25780 131095..131739 - 645 WP_000633911.1 AAA family ATPase -
DAECLI1_RS25785 131968..132939 + 972 WP_001103694.1 hypothetical protein -
DAECLI1_RS25790 132944..133336 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
DAECLI1_RS25795 133341..134611 - 1271 Protein_155 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..169561 169561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T32266 WP_001159871.1 NZ_AP018785:129573-129878 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T32266 NZ_AP018785:129573-129878 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT32266 WP_000813634.1 NZ_AP018785:129353-129571 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT32266 NZ_AP018785:129353-129571 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References