Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4023583..4024237 Replicon chromosome
Accession NZ_AP018784
Organism Escherichia coli strain SK1144

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag DAECLI1_RS19865 Protein ID WP_000244781.1
Coordinates 4023583..4023990 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag DAECLI1_RS19870 Protein ID WP_000354046.1
Coordinates 4023971..4024237 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DAECLI1_RS19845 4019540..4021273 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
DAECLI1_RS19850 4021279..4021989 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
DAECLI1_RS19855 4022014..4022910 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
DAECLI1_RS19860 4023022..4023543 + 522 WP_001055874.1 flavodoxin FldB -
DAECLI1_RS19865 4023583..4023990 - 408 WP_000244781.1 protein YgfX Toxin
DAECLI1_RS19870 4023971..4024237 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
DAECLI1_RS19875 4024480..4025460 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
DAECLI1_RS19880 4025537..4026196 - 660 WP_000250274.1 hemolysin III family protein -
DAECLI1_RS19885 4026360..4026671 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
DAECLI1_RS19890 4026716..4028149 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T32258 WP_000244781.1 NZ_AP018784:c4023990-4023583 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T32258 NZ_AP018784:c4023990-4023583 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT32258 WP_000354046.1 NZ_AP018784:c4024237-4023971 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT32258 NZ_AP018784:c4024237-4023971 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTTTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References