Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3091386..3092217 Replicon chromosome
Accession NZ_AP018784
Organism Escherichia coli strain SK1144

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag DAECLI1_RS15625 Protein ID WP_000854815.1
Coordinates 3091843..3092217 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag DAECLI1_RS15620 Protein ID WP_001280918.1
Coordinates 3091386..3091754 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DAECLI1_RS15575 3086977..3087654 + 678 WP_001362823.1 hypothetical protein -
DAECLI1_RS15580 3087670..3088080 + 411 WP_000846703.1 hypothetical protein -
DAECLI1_RS15590 3088301..3089119 + 819 WP_001542275.1 DUF945 domain-containing protein -
DAECLI1_RS15595 3089119..3089364 + 246 WP_001164966.1 hypothetical protein -
DAECLI1_RS15600 3089458..3089931 + 474 WP_001542276.1 antirestriction protein -
DAECLI1_RS15605 3089947..3090423 + 477 WP_001186200.1 RadC family protein -
DAECLI1_RS15610 3090486..3090707 + 222 WP_000692345.1 DUF987 domain-containing protein -
DAECLI1_RS15615 3090726..3091370 + 645 WP_000086752.1 hypothetical protein -
DAECLI1_RS15620 3091386..3091754 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
DAECLI1_RS15625 3091843..3092217 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
DAECLI1_RS15630 3092214..3092408 + 195 WP_000988600.1 hypothetical protein -
DAECLI1_RS15635 3092421..3092534 + 114 WP_001161660.1 DUF957 domain-containing protein -
DAECLI1_RS15640 3092823..3092969 - 147 Protein_2977 transposase domain-containing protein -
DAECLI1_RS15645 3093023..3093205 + 183 WP_001296208.1 hypothetical protein -
DAECLI1_RS15650 3093306..3093635 - 330 WP_000450409.1 DUF496 family protein -
DAECLI1_RS15655 3093807..3094865 - 1059 WP_001200889.1 FUSC family protein -
DAECLI1_RS15660 3095063..3095536 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
DAECLI1_RS15665 3095655..3096821 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T32256 WP_000854815.1 NZ_AP018784:3091843-3092217 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T32256 NZ_AP018784:3091843-3092217 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT32256 WP_001280918.1 NZ_AP018784:3091386-3091754 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT32256 NZ_AP018784:3091386-3091754 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References