Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1367926..1368544 Replicon chromosome
Accession NZ_AP018784
Organism Escherichia coli strain SK1144

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag DAECLI1_RS06605 Protein ID WP_001291435.1
Coordinates 1367926..1368144 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag DAECLI1_RS06610 Protein ID WP_000344800.1
Coordinates 1368170..1368544 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DAECLI1_RS06570 1363213..1363785 + 573 WP_000779831.1 YbaY family lipoprotein -
DAECLI1_RS06575 1363816..1364127 - 312 WP_000409908.1 MGMT family protein -
DAECLI1_RS06585 1364506..1364859 + 354 WP_000878135.1 DUF1428 family protein -
DAECLI1_RS06590 1364901..1366451 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
DAECLI1_RS06595 1366615..1367085 - 471 WP_000136192.1 YlaC family protein -
DAECLI1_RS06600 1367201..1367752 - 552 WP_000102539.1 maltose O-acetyltransferase -
DAECLI1_RS06605 1367926..1368144 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
DAECLI1_RS06610 1368170..1368544 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
DAECLI1_RS06615 1369090..1372239 - 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
DAECLI1_RS06620 1372262..1373455 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T32241 WP_001291435.1 NZ_AP018784:c1368144-1367926 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T32241 NZ_AP018784:c1368144-1367926 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT32241 WP_000344800.1 NZ_AP018784:c1368544-1368170 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT32241 NZ_AP018784:c1368544-1368170 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References