Detailed information of TA system TA032239

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 750450..751282
• Replicon: chromosome
• Accession: NZ_AP018784
• Organism: Escherichia coli strain SK1144

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: DAECLI1_RS03700
• Protein ID: WP_000854765.1
• Coordinates: 750908..751282 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: DAECLI1_RS03695
• Protein ID: WP_001295723.1
• Coordinates: 750450..750818 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DAECLI1_RS03660	747286..747741	+	456	WP_000581504.1	hypothetical protein	-
DAECLI1_RS03665	747820..748053	+	234	WP_001119729.1	DUF905 family protein	-
DAECLI1_RS03675	748153..748971	+	819	WP_001234620.1	DUF945 domain-containing protein	-
DAECLI1_RS03680	749026..749511	+	486	WP_000849588.1	antirestriction protein	-
DAECLI1_RS03685	749527..750003	+	477	WP_061089841.1	RadC family protein	-
DAECLI1_RS03690	750066..750287	+	222	WP_000692311.1	DUF987 domain-containing protein	-
DAECLI1_RS03695	750450..750818	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DAECLI1_RS03700	750908..751282	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
DAECLI1_RS03705	751279..751770	+	492	WP_000976842.1	hypothetical protein	-
DAECLI1_RS03710	751787..751963	+	177	WP_000839290.1	DUF957 domain-containing protein	-
DAECLI1_RS26335	752063..752221	+	159	WP_001467148.1	hypothetical protein	-
DAECLI1_RS03720	752597..753294	-	698	Protein_682	IS1 family transposase	-
DAECLI1_RS03725	753349..753519	+	171	Protein_683	fimbrial protein	-
DAECLI1_RS03730	753693..755036	-	1344	WP_000558251.1	gluconate permease GntP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T32239 WP_000854765.1 NZ_AP018784:750908-751282 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T32239 NZ_AP018784:750908-751282 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT32239 WP_001295723.1 NZ_AP018784:750450-750818 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT32239 NZ_AP018784:750450-750818 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCAGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52