Detailed information of TA system TA032232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 759351..759988
• Replicon: chromosome
• Accession: NZ_AP018783
• Organism: Haemophilus influenzae strain CHBN-V-3 isolate 15H03

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: E4QWH2
• Locus tag: K6J71_RS03840
• Protein ID: WP_005649049.1
• Coordinates: 759351..759755 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: E4QWH3
• Locus tag: K6J71_RS03845
• Protein ID: WP_005649046.1
• Coordinates: 759752..759988 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J71_RS03810 (CHBNV3_07310)	754907..755473	+	567	WP_005544066.1	elongation factor P	-
K6J71_RS03820 (CHBNV3_07320)	755821..756414	-	594	WP_005666656.1	primosomal replication protein	-
K6J71_RS03825 (CHBNV3_07330)	756447..757799	-	1353	WP_005649062.1	Na+/H+ antiporter family protein	-
K6J71_RS03830 (CHBNV3_07340)	758114..758803	-	690	WP_005649060.1	ribonuclease T	-
K6J71_RS03835 (CHBNV3_07350)	758877..759284	-	408	WP_005654069.1	lactoylglutathione lyase	-
K6J71_RS03840 (CHBNV3_07360)	759351..759755	-	405	WP_005649049.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6J71_RS03845 (CHBNV3_07370)	759752..759988	-	237	WP_005649046.1	antitoxin	Antitoxin
K6J71_RS03850 (CHBNV3_07380)	760081..760806	-	726	WP_005649042.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
K6J71_RS03855 (CHBNV3_07390)	760859..761377	-	519	WP_005649040.1	isoprenylcysteine carboxyl methyltransferase family protein	-
K6J71_RS03860 (CHBNV3_07400)	761596..763362	+	1767	WP_005649039.1	aspartate--tRNA ligase	-
K6J71_RS03865 (CHBNV3_07410)	763384..763857	+	474	WP_005666660.1	dihydroneopterin triphosphate diphosphatase	-
K6J71_RS03870 (CHBNV3_07420)	763869..764609	+	741	WP_005649034.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15692.29 Da
• Isoelectric Point: 8.9777

>T32232 WP_005649049.1 NZ_AP018783:c759755-759351 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Nucleotide

• Length: 405 bp

>T32232 NZ_AP018783:c759755-759351 [Haemophilus influenzae]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGATCGCTTGTCACGCATT
GAGTTTAAATGCCGTTCTTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.19 Da
• Isoelectric Point: 4.2342

>AT32232 WP_005649046.1 NZ_AP018783:c759988-759752 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT32232 NZ_AP018783:c759988-759752 [Haemophilus influenzae]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Structures

Toxin

• AlphaFold DB: E4QWH2

Antitoxin

• AlphaFold DB: E4QWH3