Detailed information of TA system TA032230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 431860..432331
• Replicon: chromosome
• Accession: NZ_AP018783
• Organism: Haemophilus influenzae strain CHBN-V-3 isolate 15H03

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: A0A806DTA9
• Locus tag: K6J71_RS02180
• Protein ID: WP_005654546.1
• Coordinates: 431860..432042 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: K6J71_RS02185
• Protein ID: WP_005650837.1
• Coordinates: 432035..432331 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J71_RS02175 (CHBNV3_04170)	428611..431589	+	2979	WP_221282175.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
K6J71_RS02180 (CHBNV3_04180)	431860..432042	+	183	WP_005654546.1	shikimate 5-dehydrogenase	Toxin
K6J71_RS02185 (CHBNV3_04190)	432035..432331	+	297	WP_005650837.1	helix-turn-helix transcriptional regulator	Antitoxin
K6J71_RS02190 (CHBNV3_04200)	432488..434404	+	1917	WP_114877247.1	ABC transporter ATP-binding protein	-
K6J71_RS02195 (CHBNV3_04210)	434414..434950	+	537	WP_103708099.1	type I DNA topoisomerase	-
K6J71_RS02200 (CHBNV3_04220)	434966..435517	+	552	WP_103708098.1	Sua5/YciO/YrdC/YwlC family protein	-
K6J71_RS02205 (CHBNV3_04230)	435521..436327	+	807	WP_221282176.1	shikimate dehydrogenase	-
K6J71_RS02210 (CHBNV3_04240)	436339..437091	+	753	WP_005667608.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7318.33 Da
• Isoelectric Point: 6.3057

>T32230 WP_005654546.1 NZ_AP018783:431860-432042 [Haemophilus influenzae]
MYEILFYRDQNDIEPVKEYLLSLAQNESKDSRIKLNKIRDYVIQTAQQRLSELKERLKNE

Nucleotide

• Length: 183 bp

>T32230 NZ_AP018783:431860-432042 [Haemophilus influenzae]
ATGTACGAGATTCTCTTTTACAGAGACCAAAATGACATTGAGCCAGTGAAAGAGTATCTGCTATCTCTGGCTCAAAATGA
AAGCAAAGATAGTCGAATTAAACTGAATAAAATTCGAGATTATGTAATCCAAACAGCCCAACAACGCTTAAGTGAATTAA
AAGAGAGATTGAAAAATGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11092.94 Da
• Isoelectric Point: 10.3139

>AT32230 WP_005650837.1 NZ_AP018783:432035-432331 [Haemophilus influenzae]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRVKNA

Nucleotide

• Length: 297 bp

>AT32230 NZ_AP018783:432035-432331 [Haemophilus influenzae]
ATGAATAAAATCAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGAGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGCCAGCAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAGCTTGAAACGTTGTTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTTCCTCTAAGGGTAAAAAATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A806DTA9