Detailed information of TA system TA032224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 953368..953974
• Replicon: chromosome
• Accession: NZ_AP018782
• Organism: Haemophilus influenzae strain CHBN-V-2 isolate 13H37

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2V0SD64
• Locus tag: K6J59_RS04765
• Protein ID: WP_005667742.1
• Coordinates: 953368..953544 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A806ED58
• Locus tag: K6J59_RS04770
• Protein ID: WP_005642973.1
• Coordinates: 953573..953974 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J59_RS08900	948373..949146	+	774	WP_246589504.1	hypothetical protein	-
K6J59_RS04735 (CHBNV2_08970)	949165..949533	+	369	WP_015979412.1	hypothetical protein	-
K6J59_RS04740 (CHBNV2_08980)	949533..949718	+	186	WP_005667755.1	hypothetical protein	-
K6J59_RS04745 (CHBNV2_08990)	949730..950011	+	282	WP_061398483.1	hypothetical protein	-
K6J59_RS04750 (CHBNV2_09000)	950089..952419	+	2331	WP_238144957.1	replication endonuclease	-
K6J59_RS04755 (CHBNV2_09010)	952431..952742	+	312	WP_005667746.1	hypothetical protein	-
K6J59_RS04760 (CHBNV2_09020)	952752..953270	+	519	WP_075985607.1	phage N-6-adenine-methyltransferase	-
K6J59_RS04765 (CHBNV2_09030)	953368..953544	+	177	WP_005667742.1	type II toxin-antitoxin system HicA family toxin	Toxin
K6J59_RS04770 (CHBNV2_09040)	953573..953974	+	402	WP_005642973.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K6J59_RS04775 (CHBNV2_09050)	954002..954262	-	261	WP_075985638.1	ogr/Delta-like zinc finger family protein	-
K6J59_RS04780 (CHBNV2_09060)	954343..955380	-	1038	WP_005667739.1	phage portal protein	-
K6J59_RS04785 (CHBNV2_09070)	955370..957193	-	1824	WP_110435034.1	terminase ATPase subunit family protein	-
K6J59_RS04790 (CHBNV2_09080)	957396..958289	+	894	WP_110435043.1	GPO family capsid scaffolding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	935295..976016	40721

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6632.65 Da
• Isoelectric Point: 10.3659

>T32224 WP_005667742.1 NZ_AP018782:953368-953544 [Haemophilus influenzae]
VDSKTAIKMIEEDGWYLDRVKGSHHQYKHPTKKGTVTIPHPRKDLGHLEKSIKKQAGL

Nucleotide

• Length: 177 bp

>T32224 NZ_AP018782:953368-953544 [Haemophilus influenzae]
GTGGATAGCAAAACAGCAATAAAAATGATAGAAGAGGACGGTTGGTATTTAGATAGAGTTAAAGGTAGTCATCATCAATA
CAAACATCCTACAAAAAAGGGAACGGTCACGATTCCCCACCCAAGGAAAGACTTGGGGCATTTAGAAAAAAGCATTAAAA
AACAAGCGGGGCTGTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14922.06 Da
• Isoelectric Point: 4.3937

>AT32224 WP_005642973.1 NZ_AP018782:953573-953974 [Haemophilus influenzae]
MLYPICIEKVNDGYVVSVPDVPGCFSAGDTLSEAMLNAKEAISFHIEGMLEDDEELPKSNPIEQYINQPEYKDFIVTVVD
VDLTHLMGKAEKINITVPALLLHRIDQFIATHPEYKNRSNFLSQLATNRLLSA

Nucleotide

• Length: 402 bp

>AT32224 NZ_AP018782:953573-953974 [Haemophilus influenzae]
ATGTTATACCCAATTTGTATCGAAAAAGTAAATGATGGTTATGTGGTATCTGTGCCAGATGTGCCAGGCTGTTTTTCTGC
TGGGGATACCTTATCAGAAGCAATGTTAAACGCAAAAGAAGCAATCTCTTTTCACATTGAAGGGATGTTAGAAGATGATG
AAGAATTGCCTAAATCTAACCCAATAGAACAATATATTAATCAGCCCGAATATAAAGATTTTATTGTCACTGTTGTTGAT
GTAGATTTAACTCATTTAATGGGTAAGGCAGAAAAAATTAATATTACGGTTCCTGCGTTATTACTGCACCGCATTGATCA
GTTTATTGCCACCCATCCAGAATATAAAAATAGAAGTAACTTTTTGTCTCAACTAGCAACAAATAGATTACTTTCTGCAT
AA

Structures

Toxin

• AlphaFold DB: A0A2V0SD64

Antitoxin

• AlphaFold DB: A0A806ED58