Detailed information of TA system TA032223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 725727..726364
• Replicon: chromosome
• Accession: NZ_AP018782
• Organism: Haemophilus influenzae strain CHBN-V-2 isolate 13H37

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: E4QWH2
• Locus tag: K6J59_RS03605
• Protein ID: WP_005649049.1
• Coordinates: 725727..726131 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: E4QWH3
• Locus tag: K6J59_RS03610
• Protein ID: WP_005649046.1
• Coordinates: 726128..726364 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J59_RS03575 (CHBNV2_06760)	721283..721849	+	567	WP_005544066.1	elongation factor P	-
K6J59_RS03585 (CHBNV2_06770)	722197..722790	-	594	WP_005666656.1	primosomal replication protein	-
K6J59_RS03590 (CHBNV2_06780)	722823..724175	-	1353	WP_005649062.1	Na+/H+ antiporter family protein	-
K6J59_RS03595 (CHBNV2_06790)	724490..725179	-	690	WP_005649060.1	ribonuclease T	-
K6J59_RS03600 (CHBNV2_06800)	725253..725660	-	408	WP_005654069.1	lactoylglutathione lyase	-
K6J59_RS03605 (CHBNV2_06810)	725727..726131	-	405	WP_005649049.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6J59_RS03610 (CHBNV2_06820)	726128..726364	-	237	WP_005649046.1	antitoxin	Antitoxin
K6J59_RS03615 (CHBNV2_06830)	726457..727182	-	726	WP_005649042.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
K6J59_RS03620 (CHBNV2_06840)	727235..727753	-	519	WP_005649040.1	isoprenylcysteine carboxyl methyltransferase family protein	-
K6J59_RS03625 (CHBNV2_06850)	727972..729738	+	1767	WP_005649039.1	aspartate--tRNA ligase	-
K6J59_RS03630 (CHBNV2_06860)	729760..730233	+	474	WP_005666660.1	dihydroneopterin triphosphate diphosphatase	-
K6J59_RS03635 (CHBNV2_06870)	730245..730985	+	741	WP_005649034.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15692.29 Da
• Isoelectric Point: 8.9777

>T32223 WP_005649049.1 NZ_AP018782:c726131-725727 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Nucleotide

• Length: 405 bp

>T32223 NZ_AP018782:c726131-725727 [Haemophilus influenzae]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGATCGCTTGTCACGCATT
GAGTTTAAATGCCGTTCTTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.19 Da
• Isoelectric Point: 4.2342

>AT32223 WP_005649046.1 NZ_AP018782:c726364-726128 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT32223 NZ_AP018782:c726364-726128 [Haemophilus influenzae]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Structures

Toxin

• AlphaFold DB: E4QWH2

Antitoxin

• AlphaFold DB: E4QWH3