Detailed information of TA system TA032221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 398369..398840
• Replicon: chromosome
• Accession: NZ_AP018782
• Organism: Haemophilus influenzae strain CHBN-V-2 isolate 13H37

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: A0A806DTA9
• Locus tag: K6J59_RS01945
• Protein ID: WP_005654546.1
• Coordinates: 398369..398551 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: K6J59_RS01950
• Protein ID: WP_005650837.1
• Coordinates: 398544..398840 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J59_RS01940 (CHBNV2_03640)	395025..398099	+	3075	WP_221276220.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
K6J59_RS01945 (CHBNV2_03650)	398369..398551	+	183	WP_005654546.1	shikimate 5-dehydrogenase	Toxin
K6J59_RS01950 (CHBNV2_03660)	398544..398840	+	297	WP_005650837.1	helix-turn-helix transcriptional regulator	Antitoxin
K6J59_RS01955 (CHBNV2_03670)	399024..400940	+	1917	WP_020910212.1	ABC transporter ATP-binding protein	-
K6J59_RS01960 (CHBNV2_03680)	400950..401486	+	537	WP_020910213.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
K6J59_RS01965 (CHBNV2_03690)	401502..402053	+	552	WP_020910214.1	Sua5/YciO/YrdC/YwlC family protein	-
K6J59_RS01970 (CHBNV2_03700)	402057..402863	+	807	WP_020910215.1	shikimate dehydrogenase	-
K6J59_RS01975 (CHBNV2_03710)	402875..403627	+	753	WP_020910216.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7318.33 Da
• Isoelectric Point: 6.3057

>T32221 WP_005654546.1 NZ_AP018782:398369-398551 [Haemophilus influenzae]
MYEILFYRDQNDIEPVKEYLLSLAQNESKDSRIKLNKIRDYVIQTAQQRLSELKERLKNE

Nucleotide

• Length: 183 bp

>T32221 NZ_AP018782:398369-398551 [Haemophilus influenzae]
ATGTACGAGATTCTCTTTTACAGAGACCAAAATGACATTGAGCCAGTGAAAGAATATCTGCTATCGCTGGCTCAAAATGA
AAGCAAAGATAGTCGAATTAAACTGAATAAAATTCGAGATTATGTAATCCAAACAGCCCAACAACGCTTAAGTGAATTAA
AAGAGAGATTGAAAAATGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11092.94 Da
• Isoelectric Point: 10.3139

>AT32221 WP_005650837.1 NZ_AP018782:398544-398840 [Haemophilus influenzae]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRVKNA

Nucleotide

• Length: 297 bp

>AT32221 NZ_AP018782:398544-398840 [Haemophilus influenzae]
ATGAATAAAATTAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGAGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGCCAACAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAGCTTGAAACGTTGTTAAAATTG
CTCGCTCCTTTAGGAAAAACCCTGTCGATTGTGCCTCTAAGGGTAAAAAATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A806DTA9