Detailed information of TA system TA032219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 46627..47258
• Replicon: chromosome
• Accession: NZ_AP018782
• Organism: Haemophilus influenzae strain CHBN-V-2 isolate 13H37

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K6J59_RS00225
• Protein ID: WP_005648013.1
• Coordinates: 46860..47258 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q4QLV9
• Locus tag: K6J59_RS00220
• Protein ID: WP_005648011.1
• Coordinates: 46627..46860 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J59_RS00195 (CHBNV2_00390)	42105..43307	-	1203	WP_005666020.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
K6J59_RS00200 (CHBNV2_00400)	43471..44136	+	666	WP_020910156.1	DNA repair protein RadC	-
K6J59_RS00205 (CHBNV2_00410)	44350..44586	+	237	WP_005542826.1	50S ribosomal protein L28	-
K6J59_RS00210 (CHBNV2_00420)	44598..44768	+	171	WP_005613503.1	50S ribosomal protein L33	-
K6J59_RS00215 (CHBNV2_00430)	45116..46480	+	1365	WP_005648009.1	diaminobutyrate--2-oxoglutarate transaminase	-
K6J59_RS00220 (CHBNV2_00440)	46627..46860	+	234	WP_005648011.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
K6J59_RS00225 (CHBNV2_00450)	46860..47258	+	399	WP_005648013.1	tRNA(fMet)-specific endonuclease VapC	Toxin
K6J59_RS00230 (CHBNV2_00460)	47278..48813	+	1536	WP_005648022.1	L-2,4-diaminobutyrate decarboxylase	-
K6J59_RS00235 (CHBNV2_00470)	49045..49860	+	816	WP_005666022.1	DNA-formamidopyrimidine glycosylase	-
K6J59_RS00240 (CHBNV2_00480)	49934..50953	-	1020	WP_005666023.1	outer membrane-stress sensor serine endopeptidase DegS	-
K6J59_RS00245 (CHBNV2_00490)	50954..52072	-	1119	WP_005666024.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15073.38 Da
• Isoelectric Point: 8.0514

>T32219 WP_005648013.1 NZ_AP018782:46860-47258 [Haemophilus influenzae]
VLKYMLDTNIVIYVIKRRPLEILSRFNQNAGKMCVSSITVAELYYGAEKSEYPERNIAGIEDFLSRLTILDYQPKHAAHF
GNIKAELSKQGKLIGENDIHIAAHARSEGLILVSNNLREFERVIALRTENWV

Nucleotide

• Length: 399 bp

>T32219 NZ_AP018782:46860-47258 [Haemophilus influenzae]
GTGTTGAAATATATGCTTGATACTAACATTGTGATTTACGTGATTAAGCGTAGGCCATTGGAAATTCTTTCTCGCTTTAA
TCAAAATGCAGGAAAAATGTGCGTGAGTAGCATTACTGTGGCTGAACTCTATTATGGAGCTGAAAAAAGCGAATATCCCG
AAAGAAACATTGCGGGTATTGAAGATTTCTTATCACGCCTCACCATTTTAGATTACCAACCAAAGCATGCAGCTCATTTC
GGCAATATTAAAGCTGAACTCTCTAAACAAGGGAAATTGATTGGCGAAAATGATATTCATATAGCGGCTCATGCTCGTAG
TGAAGGGTTAATTCTGGTTAGTAACAATTTAAGAGAATTTGAGCGAGTTATAGCATTACGTACAGAAAATTGGGTGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8880.98 Da
• Isoelectric Point: 4.4013

>AT32219 WP_005648011.1 NZ_AP018782:46627-46860 [Haemophilus influenzae]
MIEASVFMTNRSQAVRLPAEVRFSEEIKKLSVRVSGSDRILSPLNQSWDSFFLNDQAVSDDFMNEREIAFQPEREAL

Nucleotide

• Length: 234 bp

>AT32219 NZ_AP018782:46627-46860 [Haemophilus influenzae]
ATGATTGAAGCCTCTGTTTTTATGACTAATCGAAGTCAAGCTGTGCGTTTGCCCGCTGAAGTTCGCTTTTCTGAAGAAAT
CAAAAAATTATCGGTTCGTGTATCGGGCAGTGATCGGATTTTATCGCCTCTCAACCAATCTTGGGATAGTTTTTTCTTGA
ATGATCAAGCAGTTAGCGATGATTTTATGAATGAACGAGAAATTGCATTTCAACCAGAGCGTGAGGCTTTATAG

Structures

Antitoxin

• AlphaFold DB: A0A806DGS0