Detailed information of TA system TA032216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1074132..1074738
• Replicon: chromosome
• Accession: NZ_AP018781
• Organism: Haemophilus influenzae strain CHBN-V-1 isolate 13H43

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2V0SD64
• Locus tag: CHBNTHi_RS05420
• Protein ID: WP_005667742.1
• Coordinates: 1074132..1074308 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A806ED58
• Locus tag: CHBNTHi_RS05425
• Protein ID: WP_005642973.1
• Coordinates: 1074337..1074738 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHBNTHi_RS05385 (CHBNV1_10210)	1069151..1069336	+	186	WP_005667755.1	hypothetical protein	-
CHBNTHi_RS05390 (CHBNV1_10220)	1069348..1069629	+	282	WP_015967401.1	hypothetical protein	-
CHBNTHi_RS05395 (CHBNV1_10230)	1069707..1072037	+	2331	WP_260181958.1	replication endonuclease	-
CHBNTHi_RS05400 (CHBNV1_10240)	1072049..1072357	+	309	WP_110437526.1	hypothetical protein	-
CHBNTHi_RS05405 (CHBNV1_10250)	1072354..1072893	+	540	WP_238144848.1	DNA cytosine methyltransferase	-
CHBNTHi_RS05410 (CHBNV1_10260)	1072906..1073751	+	846	WP_110437525.1	hypothetical protein	-
CHBNTHi_RS05415 (CHBNV1_10270)	1073845..1074036	+	192	WP_238144847.1	phage N-6-adenine-methyltransferase	-
CHBNTHi_RS05420 (CHBNV1_10280)	1074132..1074308	+	177	WP_005667742.1	type II toxin-antitoxin system HicA family toxin	Toxin
CHBNTHi_RS05425 (CHBNV1_10290)	1074337..1074738	+	402	WP_005642973.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CHBNTHi_RS05430 (CHBNV1_10300)	1074765..1075025	-	261	WP_071819916.1	ogr/Delta-like zinc finger family protein	-
CHBNTHi_RS05435 (CHBNV1_10310)	1075104..1076141	-	1038	WP_005667739.1	phage portal protein	-
CHBNTHi_RS05440 (CHBNV1_10320)	1076131..1077954	-	1824	WP_015967402.1	terminase ATPase subunit family protein	-
CHBNTHi_RS05445 (CHBNV1_10330)	1078158..1079051	+	894	WP_005667735.1	GPO family capsid scaffolding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1065250..1096409	31159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6632.65 Da
• Isoelectric Point: 10.3659

>T32216 WP_005667742.1 NZ_AP018781:1074132-1074308 [Haemophilus influenzae]
VDSKTAIKMIEEDGWYLDRVKGSHHQYKHPTKKGTVTIPHPRKDLGHLEKSIKKQAGL

Nucleotide

• Length: 177 bp

>T32216 NZ_AP018781:1074132-1074308 [Haemophilus influenzae]
GTGGATAGCAAAACAGCAATAAAAATGATAGAAGAGGACGGTTGGTATTTAGATAGAGTTAAAGGTAGTCATCATCAATA
CAAACATCCTACAAAAAAGGGAACGGTCACGATTCCCCACCCAAGGAAAGACTTGGGGCATTTAGAAAAAAGCATTAAAA
AACAAGCGGGGCTGTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14922.06 Da
• Isoelectric Point: 4.3937

>AT32216 WP_005642973.1 NZ_AP018781:1074337-1074738 [Haemophilus influenzae]
MLYPICIEKVNDGYVVSVPDVPGCFSAGDTLSEAMLNAKEAISFHIEGMLEDDEELPKSNPIEQYINQPEYKDFIVTVVD
VDLTHLMGKAEKINITVPALLLHRIDQFIATHPEYKNRSNFLSQLATNRLLSA

Nucleotide

• Length: 402 bp

>AT32216 NZ_AP018781:1074337-1074738 [Haemophilus influenzae]
ATGTTATACCCGATTTGTATCGAAAAAGTAAATGATGGTTATGTGGTATCTGTGCCAGATGTGCCAGGCTGTTTTTCTGC
TGGGGATACCTTATCAGAAGCGATGTTAAACGCAAAAGAAGCAATCTCTTTTCACATTGAAGGGATGTTAGAAGATGATG
AAGAATTGCCTAAATCTAACCCAATAGAACAATATATTAATCAGCCCGAATATAAAGATTTTATTGTCACTGTTGTTGAT
GTAGATTTAACTCATTTAATGGGTAAGGCAGAAAAAATTAATATTACGGTTCCAGCGTTATTACTGCACCGCATTGATCA
GTTTATTGCCACCCATCCAGAATATAAGAATAGAAGTAACTTTTTGTCTCAACTAGCAACAAATAGATTACTTTCTGCAT
AA

Structures

Toxin

• AlphaFold DB: A0A2V0SD64

Antitoxin

• AlphaFold DB: A0A806ED58