Detailed information of TA system TA032213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 402506..402977
• Replicon: chromosome
• Accession: NZ_AP018781
• Organism: Haemophilus influenzae strain CHBN-V-1 isolate 13H43

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: A0A806DTA9
• Locus tag: CHBNTHi_RS01970
• Protein ID: WP_005654546.1
• Coordinates: 402506..402688 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: CHBNTHi_RS01975
• Protein ID: WP_061671753.1
• Coordinates: 402681..402977 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHBNTHi_RS01960 (CHBNV1_03690)	398692..398856	-	165	WP_050848769.1	hypothetical protein	-
CHBNTHi_RS01965 (CHBNV1_03700)	399165..402236	+	3072	WP_221260931.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
CHBNTHi_RS01970 (CHBNV1_03710)	402506..402688	+	183	WP_005654546.1	shikimate 5-dehydrogenase	Toxin
CHBNTHi_RS01975 (CHBNV1_03720)	402681..402977	+	297	WP_061671753.1	helix-turn-helix transcriptional regulator	Antitoxin
CHBNTHi_RS01980 (CHBNV1_03730)	403161..405077	+	1917	WP_110437457.1	ABC transporter ATP-binding protein	-
CHBNTHi_RS01985 (CHBNV1_03740)	405087..405623	+	537	WP_053521035.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
CHBNTHi_RS01990 (CHBNV1_03750)	405639..406190	+	552	WP_110437456.1	Sua5/YciO/YrdC/YwlC family protein	-
CHBNTHi_RS01995 (CHBNV1_03760)	406194..407012	+	819	WP_110437455.1	shikimate dehydrogenase	-
CHBNTHi_RS02000 (CHBNV1_03770)	407009..407566	+	558	WP_110437454.1	DNA-3-methyladenine glycosylase I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7318.33 Da
• Isoelectric Point: 6.3057

>T32213 WP_005654546.1 NZ_AP018781:402506-402688 [Haemophilus influenzae]
MYEILFYRDQNDIEPVKEYLLSLAQNESKDSRIKLNKIRDYVIQTAQQRLSELKERLKNE

Nucleotide

• Length: 183 bp

>T32213 NZ_AP018781:402506-402688 [Haemophilus influenzae]
ATGTACGAGATTCTCTTTTACAGAGACCAAAATGACATTGAGCCAGTGAAAGAATATCTGCTATCGCTGGCTCAAAATGA
AAGCAAAGATAGTCGAATTAAACTGAATAAAATTCGAGATTATGTAATCCAAACTGCCCAACAACGCTTAAGTGAATTAA
AAGAGAGATTGAAAAATGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11106.97 Da
• Isoelectric Point: 10.3139

>AT32213 WP_061671753.1 NZ_AP018781:402681-402977 [Haemophilus influenzae]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRIKNA

Nucleotide

• Length: 297 bp

>AT32213 NZ_AP018781:402681-402977 [Haemophilus influenzae]
ATGAATAAAATCAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGCGAAAG
TAATTTACGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGCCAGCAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAACTTGAAACGTTATTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTGCCTCTAAGGATAAAAAATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A806DTA9